Gold Nanoparticles-Based Colorimetric Assay for Cathepsin B Activity and the Efficiency of Its Inhibitors

被引:39
|
作者
Kim, Chan-Jin [1 ]
Lee, Dong-Ik [2 ]
Kim, Cheonghee [1 ]
Lee, Kangtaek [1 ]
Lee, Chang-Ha [1 ]
Ahn, Ik-Sung [1 ]
机构
[1] Yonsei Univ, Dept Chem & Biomol Engn, Seoul 120749, South Korea
[2] Dityrosine Innovat Chem DI Chem, Seoul 120749, South Korea
关键词
CYSTEINE CATHEPSINS; AU NANOPARTICLES; PROTEASES; SPECIFICITY; IDENTIFICATION; DITYROSINE; STRATEGY; GROWTH; SIZE;
D O I
10.1021/ac4039064
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cathepsin B has been suggested to be a prognostic marker of melanoma, glioma, and a variety of cancers such as brain, breast, colon, esophageal, gastric, lung, ovarian, and thyroid cancers. Cathepsin B inhibitors have also been considered as anticancer drug candidates; hence, there has been a growing need for a probe which enables the selective and simple detection of cathepsin B and, its inhibitors. For the purpose of selective assay, a cathepsin B-specific substrate, N,N'-diBoc-dityrosine-glycine-phenylalanine-3-(methylthio)-propylamine (DBDY-Gly-Phe-MTPA) was synthesized in this study. Phe-MTPA, which was produced via cathepsin B-catalyzed hydrolysis of DBDY-Gly-Phe-MTPA, allowed aggregation of gold nanoparticles (AuNPs) leading to a color change from red to blue. When tested for cathepsins B, L, and S, this assay method exhibited AuNPs color change only in reaction to cathepsin B. The limits of detection for cathepsin B was 10 and 5 nM in the 1 and 2 h hydrolysis reactions, respectively. The efficiency of cathepsin B inhibitors such as leupeptin, antipain, and chymostatin was easily compared by the degree of color change. Moreover, IC50 values of leupeptin, antipain, and chymostatin were found to be 0.11, 0.48, and 1.78 mu M, respectively, which were similar to the results of previous studies. Therefore the colorimetric assay of cathepsin B and cathepsin B inhibitors using DBDY-Gly-Phe-MTPA and AuNPs allowed not only the selective but also the simple assay of cathepsin B and its inhibitors, which was possible with the naked eye.
引用
收藏
页码:3825 / 3833
页数:9
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