Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells

被引：26

作者：

Li, Yumeng ^{[1
]}

Wang, Shushu ^{[1
]}

Chen, Yanchi ^{[1
]}

Li, Manjia ^{[1
]}

Dong, Xiaoshu ^{[1
]}

Hang, Howard C. ^{[2
,3
,4
]}

Peng, Tao ^{[1
]}

机构：

[1] Peking Univ, Sch Chem Biol & Biotechnol, State Key Lab Chem Oncogen, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China

[2] Rockefeller Univ, Lab Chem Biol & Microbial Pathogenesis, New York, NY 10065 USA

[3] Scripps Res, Dept Immunol & Microbiol, La Jolla, CA 92037 USA

[4] Scripps Res, Dept Chem, La Jolla, CA 92037 USA

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 89期

基金：

中国国家自然科学基金;

关键词：

DIELS-ALDER REACTIONS; MAMMALIAN-CELLS; RAS; LIGATION;

D O I：

10.1039/d0cc06073a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein S-palmitoylation, or S-fatty-acylation, regulates many fundamental cellular processes in eukaryotes. Herein, we present a chemical fatty-acylation approach that involves site-specific incorporation of cycloalkyne-containing unnatural amino acids and subsequent bioorthogonal reactions with fatty-acyl tetrazines to install fatty-acylation mimics at target protein sites, allowing gain-of-function analysis of S-palmitoylation in live cells.

引用

页码：13880 / 13883

页数：4

共 38 条

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