How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights

被引:14
|
作者
Costa, Viviana [1 ]
De Fine, Marcello [2 ]
Carina, Valeria [1 ]
Conigliaro, Alice [3 ]
Raimondi, Lavinia [1 ]
De Luca, Angela [1 ]
Bellavia, Daniele [1 ]
Salamanna, Francesca [1 ]
Alessandro, Riccardo [3 ,4 ]
Pignatti, Giovanni [2 ]
Fini, Milena [1 ]
Giavaresi, Gianluca [1 ]
机构
[1] IRCCS Ist Ortoped Rizzoli, SC Sci & Tecnol Chirurg, SS Piattaforma Sci Om Ortopedia Personalizzata, I-40136 Bologna, Italy
[2] IRCCS Ist Ortoped Rizzoli, I-40136 Bologna, Italy
[3] Univ Palermo, Sect Biol & Genet, Dept Biomed Neurosci & Adv Diagnost BiND, I-90133 Palermo, Italy
[4] Ist Ric & Innovaz Biomed IRIB, I-90133 Palermo, Italy
关键词
osteoarthritis; microRNAs; osteoblasts; chondrocytes; SP1; CX43;
D O I
10.3390/ijms22052471
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Osteoarthritis (OA) is a degenerative bone disease that involved micro and macro-environment of joints. To date, there are no radical curative treatments for OA and novel therapies are mandatory. Recent evidence suggests the role of miRNAs in OA progression. In our previous studies, we demonstrated the role of miR-31-5p and miR-33a families in different bone regeneration signaling. Here, we investigated the role of miR-31-5p and miR-33a-5p in OA progression. A different expression of miR-31-5p and miR-33a-5p into osteoblasts and chondrocytes isolated from joint tissues of OA patients classified in based on different Kellgren and Lawrence (KL) grading was highlighted; and through a bioinformatic approach the common miRNAs target Specificity proteins (Sp1) were identified. Sp1 regulates the expression of gap junction protein Connexin43 (Cx43), which in OA drives the modification of (i) osteoblasts and chondrocytes genes expression, (ii) joint inflammation cytokines releases and (iii) cell functions. Concerning this, thanks to gain and loss of function studies, the possible role of Sp1 as a modulator of CX43 expression through miR-31-5p and miR-33a-5p action was also evaluated. Finally, we hypothesize that both miRNAs cooperate to modulate the expression of SP1 in osteoblasts and chondrocytes and interfering, consequently, with CX43 expression, and they might be further investigated as new possible biomarkers for OA.
引用
收藏
页码:1 / 16
页数:15
相关论文
共 50 条
  • [21] HIF1α-SP1 interaction disrupts the circ-0001875/miR-31-5p/SP1 regulatory loop under a hypoxic microenvironment and promotes non-small cell lung cancer progression
    Dong Wu
    Tingting Chen
    Xuanna Zhao
    Dan Huang
    Jiawei Huang
    Yujie Huang
    Qiu Huang
    Zhu Liang
    Chunyuan Chen
    Min Chen
    Dongming Li
    Bin Wu
    Lixia Li
    Journal of Experimental & Clinical Cancer Research, 41
  • [22] miR-33a-5p modulates TNF-α-inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs
    Mi, Wenxiang
    Shi, Qiongling
    Chen, Xipeng
    Wu, Tingting
    Huang, Hui
    FEBS LETTERS, 2016, 590 (03) : 396 - 407
  • [23] Polyphyllin VII enhances the sensitivity of endometrial carcinoma cells to medroxyprogesterone acetate through upregulating miR-33a-5p expression
    Liu, Haoen
    Peng, Yan
    Zhuang, Xiaodan
    ONCOLOGY LETTERS, 2025, 29 (02)
  • [24] lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling
    Jinchang Pan
    Shuai Fang
    Haihua Tian
    Chengwei Zhou
    Xiaodong Zhao
    Hui Tian
    Jinxian He
    Weiyu Shen
    Xiaodan Meng
    Xiaofeng Jin
    Zhaohui Gong
    Molecular Cancer, 19
  • [25] lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling
    Pan, Jinchang
    Fang, Shuai
    Tian, Haihua
    Zhou, Chengwei
    Zhao, Xiaodong
    Tian, Hui
    He, Jinxian
    Shen, Weiyu
    Meng, Xiaodan
    Jin, Xiaofeng
    Gong, Zhaohui
    MOLECULAR CANCER, 2020, 19 (01)
  • [26] miR-31-5p Is a LIPUS-Mechanosensitive MicroRNA that Targets HIF-1α Signaling and Cytoskeletal Proteins
    Costa, Viviana
    Carina, Valeria
    Conigliaro, Alice
    Raimondi, Lavinia
    De Luca, Angela
    Bellavia, Daniele
    Salamanna, Francesca
    Setti, Stefania
    Alessandro, Riccardo
    Fini, Milena
    Giavaresi, Gianluca
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (07):
  • [27] Higher expression of mir-31-5p is associated with reduced risk of head and neck keloid recurrence following surgical resection
    Levin, Albert M.
    Okifo, Oghenefejiro
    Buhl, Katherine
    Ouchi, Takahiro
    Parker, Bianca
    Tan, Jessica
    Datta, Indrani
    Dai, Xiangguo
    Chen, Yalei
    Palanisamy, Nallasivam
    Veenstra, Jesse
    Carskadon, Shannon
    Li, Jia
    Ozog, David
    Keller, Christian E.
    Chitale, Dhananjay
    Bobbitt, Kevin R.
    Crawford, Howard C.
    Steele, Nina
    Mi, Qing-Sheng
    Jones, Lamont R.
    LARYNGOSCOPE INVESTIGATIVE OTOLARYNGOLOGY, 2024, 9 (06):
  • [28] MiR-33a-5p targets NOMO1 to modulate human cardiomyocyte progenitor cells proliferation and differentiation and apoptosis
    Xing, Wang
    Li, Tiangang
    Wang, Yixuan
    Qiang, Yi
    Ai, Chencheng
    Tang, Hanbo
    JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION, 2021, 41 (05) : 476 - 487
  • [29] miR-33a-5p inhibits the progression of esophageal cancer through the DKK1-mediatea Wnt/β-catenin pathway
    Song, Qingping
    Liu, Hui
    Li, Chengyan
    Liang, Haifeng
    AGING-US, 2021, 13 (16): : 20481 - 20494
  • [30] LncRNA Tincr regulates PKCe expression in a miR-31-5p-dependent manner in cardiomyocyte hypertrophy
    Li, Hao
    Shi, Hongtao
    Zhang, Fan
    Xue, Honghong
    Wang, Lei
    Tian, Jing
    Xu, Jianrong
    Han, Qinghua
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2020, 393 (12) : 2495 - 2506