CpG-containing oligodeoxynucleotides augment and switch the immune responses of cattle to bovine herpesvirus-1 glycoprotein D

被引：62
|
作者：
Rankin, R
Pontarollo, R
Gomis, S
Karvonen, B
Willson, P
Loehr, BI
Godson, DL
Babiuk, LA
Hecker, B
Little-van den Hurk, SV
机构：
[1] Univ Saskatchewan, Vet Infect Dis Org, Saskatoon, SK S7N 5E3, Canada
[2] QIAGEN GmbH, D-40724 Hilden, Germany
来源：
VACCINE | 2002年 / 20卷 / 23-24期
基金：
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词：
CpG-oligodeoxynucleotides; adjuvant; cattle;
D O I：
10.1016/S0264-410X(02)00216-5
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The adjuvanticity of a synthetic oligodeoxynucleotide containing unmethylated CpG motifs (CpG ODN) was determined in cattle. Calves were immunized with a truncated secreted version of glycoprotein D (tgD) of bovine herpes virus-1 (BHV-1) formulated with alum, CpG ODN, or a combination of both. BHV-1 tgD formulated with CpG ODN or with alum and CpG ODN induced a stronger and more balanced immune response than tgD in alum. This level of immunity was of sufficient magnitude to minimize weight loss and significantly reduce the duration of virus shedding after intranasal viral challenge. Local tissue reactions generated by CpG ODN were very mild and transient, whereas reactions induced by alum or a combination of CpG ODN and alum were moderate in severity and duration. These data demonstrate that CpG ODN causes minimal injection site reactions and yet acts as an effective adjuvant in cattle. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：3014 / 3022
页数：9
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