Effect of different nuclear localization sequences on the immune responses induced by a MIDGE vector encoding bovine herpesvirus-1 glycoprotein D

被引：23

作者：

Zheng, Chunfu

Juhls, Christiane

Oswald, Detlef

Sack, Florian

Westfehling, Ines

Wittig, Burghardt

Babiuk, Lorne A.

van Drunen Littel-van den Hurk, Sylvia

机构：

[1] Univ Saskatchewan, Vaccine & Infect Dis Org, Saskatoon, SK S7N 5E3, Canada

[2] Mologen AG, D-14195 Berlin, Germany

[3] Free Univ Berlin, Inst Mol Biol & Biochem, D-14195 Berlin, Germany

来源：

VACCINE | 2006年 / 24卷 / 21期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

MIDGE; NLS; tgD;

D O I：

10.1016/j.vaccine.2005.08.046

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To optimize the efficiency of a Minimalistic Immunogenically Defined Gene Expression (MIDGE) vector, peptides containing proven (SV40 T-antigen and bovine herpesvirus-1 VP8) or putative (herpes simplex virus-1 VP22) nuclear localization signals (NLSs) were linked to a MIDGE (R) vector encoding a truncated, secreted form of BHV-1 glycoprotein D (tgD) (MIDGE-tgD). Conjugation of an NLS to the MIDGE-tgD vector improved the tgD expression in vitro and the humoral and cellular immune responses induced in mice in vivo. The NLS from BHV-1 VP8 was most efficient at enhancing the tgD production and tgD-specific immune responses when conjugated to the MIDGE-tgD vector. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：4625 / 4629

页数：5

共 50 条

[1] Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1
Gupta, PK
Saini, M
Gupta, LK
Rao, VDP
Bandyopadhyay, SK
Butchaiah, G
Garg, GK
Garg, SK
VETERINARY MICROBIOLOGY, 2001, 78 (04) : 293 - 305
[2] EFFECT OF LEVAMISOLE IN IMMUNE-RESPONSES TO BOVINE HERPESVIRUS-1
BABIUK, LA
MISRA, V
AMERICAN JOURNAL OF VETERINARY RESEARCH, 1982, 43 (08) : 1349 - 1354
[3] Modulation of immune responses to bovine herpesvirus-1 in cattle by immunization with a DNA vaccine encoding glycoprotein D as a fusion protein with bovine CD154
Manoj, S
Griebel, PJ
Babiuk, LA
Littel-Van Den Hurk, SV
IMMUNOLOGY, 2004, 112 (02) : 328 - 338
[4] CpG-containing oligodeoxynucleotides augment and switch the immune responses of cattle to bovine herpesvirus-1 glycoprotein D
Rankin, R
Pontarollo, R
Gomis, S
Karvonen, B
Willson, P
Loehr, BI
Godson, DL
Babiuk, LA
Hecker, B
Little-van den Hurk, SV
VACCINE, 2002, 20 (23-24) : 3014 - 3022
[5] Augmentation of cellular immune responses to bovine herpesvirus-1 glycoprotein D by vaccination with CpG-enhanced plasmid vectors
Pontarollo, RA
Babiuk, LA
Hecker, R
Littel-van den Hurk, SV
JOURNAL OF GENERAL VIROLOGY, 2002, 83 : 2973 - 2981
[6] EPITOPE SPECIFICITY AND PROTECTIVE EFFICACY OF THE BOVINE IMMUNE-RESPONSE TO BOVINE HERPESVIRUS-1 GLYCOPROTEIN VACCINES
ISRAEL, BA
MARSHALL, RL
LETCHWORTH, GJ
VACCINE, 1988, 6 (04) : 349 - 356
[7] PATTERNS OF BOVINE T CELL-MEDIATED IMMUNE-RESPONSES TO BOVINE HERPESVIRUS-1
MILLEREDGE, M
SPLITTER, G
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1986, 13 (04) : 301 - 319
[8] Immune responses of mice against recombinant bovine herpesvirus 5 glycoprotein D
Dummer, Luana Alves
Araujo, Itaua Leston
Finger, Paula Fonseca
dos Santos, Alceu Goncalves, Jr.
da Rosa, Matheus Costa
Conceicao, Fabricio Rochedo
Fischer, Geferson
Littel-van den Hurk, Sylvia van Drunen
Leivas Leite, Fabio Pereira
VACCINE, 2014, 32 (21) : 2413 - 2419
[9] Cloning and Sequence Analysis of Glycoprotein D Gene of Bovine Herpesvirus-1 Strain Luojing
LI Ji-chang~1
JournalofNortheastAgriculturalUniversity(EnglishEdition), 2003, (02) : 137 - 140
[10] Immunogenicity of bovine herpesvirus 1 glycoprotein D in mice: Effect of antigen form on the induction of cellular and humoral immune responses
BacaEstrada, ME
Snider, M
Tikoo, SK
Harland, R
Babiuk, LA
LittelVandenHurk, SV
VIRAL IMMUNOLOGY, 1996, 9 (01) : 11 - 22

← 1 2 3 4 5 →