Detection of Staphylococcus aureus enterotoxin production genes from patient samples using an automated extraction platform and multiplex real-time PCR

被引：19

作者：

Chiefari, Amy K. ^{[1
]}

Perry, Michael J. ^{[1
]}

Kelly-Cirino, Cassandra ^{[1
]}

Egan, Christina T. ^{[1
]}

机构：

[1] New York State Dept Hlth, Wadsworth Ctr, Albany, NY USA

来源：

MOLECULAR AND CELLULAR PROBES | 2015年 / 29卷 / 06期

关键词：

Staphylococcus aureus; Real-time PCR; Multiplex PCR; Automated extraction; Diagnostic testing; NUCLEOTIDE-SEQUENCE; COMMON ANTIBODIES; TOXIN GENES; IDENTIFICATION; CLONING; ASSAY; C1;

D O I：

10.1016/j.mcp.2015.06.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To minimize specimen volume, handling and testing time, we have developed two TaqMar (R) multiplex real-time PCR (rtPCR) assays to detect staphylococcal enterotoxins A-E and Toxic Shock Syndrome Toxin production genes directly from clinical patient stool specimens utilizing a novel lysis extraction process in parallel with the Roche MagNA Pure Compact. These assays are specific, sensitive and reliable for the detection of the staphylococcal enterotoxin encoding genes and the tst1 gene from known toxin producing strains of Staphylococcus aureus. Specificity was determined by testing a total of 47 microorganism strains, including 8 previously characterized staphylococcal enterotoxin producing strains against each rtPCR target. Sensitivity for these assays range from 1 to 25 cfu per rtPCR reaction for cultured isolates and 8-20 cfu per rtPCR for the clinical stool matrix. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：461 / 467

页数：7

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