Pulse front adaptive optics in two-photon microscopy

被引:5
|
作者
Sun, Bangshan [1 ]
Salter, Patrick S. [1 ]
Booth, Martin J. [1 ,2 ]
机构
[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
[2] Univ Oxford, Ctr Neural Circuits & Behav, Oxford OX1 3SR, England
基金
英国工程与自然科学研究理事会;
关键词
FEMTOSECOND LASER-PULSES; FLUORESCENCE MICROSCOPY; LENS SYSTEMS; DISTORTION; ABERRATIONS; INTERFEROMETRY; COMPENSATION; RESOLUTION; IMAGE;
D O I
10.1364/OL.40.004999
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Adaptive optics has been extensively studied for the correction of phase front aberrations in optical systems. In systems using ultrafast lasers, distortions can also exist in the pulse front (contour of constant intensity in space and time), but until now their correction has been mostly unexplored due to technological limitations. In this Letter, we apply newly developed pulse front adaptive optics, for the first time to our knowledge, to practical compensation of a two-photon fluorescence microscope. With adaptive correction of the system-induced pulse front distortion, improvements beyond conventional phase correction are demonstrated. (C) 2015 Optical Society of America
引用
收藏
页码:4999 / 5002
页数:4
相关论文
共 50 条
  • [31] Adaptive optics two photon scanning laser fluorescence microscopy
    Zhou, Yaopeng
    Bifano, Thomas
    Lin, Charles
    MEMS ADAPTIVE OPTICS V, 2011, 7931
  • [32] Pulse front control with adaptive optics
    Sun, B.
    Salter, P. S.
    Booth, M. J.
    FRONTIERS IN ULTRAFAST OPTICS: BIOMEDICAL, SCIENTIFIC, AND INDUSTRIAL APPLICATIONS XVI, 2016, 9740
  • [33] Adaptive optics for in vivo two-photon calcium imaging of neuronal networks
    Meimon, Serge
    Conan, Jean-Mark
    Mugnier, Laurent M.
    Michau, Vincent
    Cossart, Rosa
    Malvache, Arnaud
    MEMS ADAPTIVE OPTICS VIII, 2014, 8978
  • [34] New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy
    Booth, MJ
    Neil, MAA
    Wilson, T
    JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2002, 19 (10) : 2112 - 2120
  • [35] Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo
    Zhongya Qin
    Sicong He
    Chao Yang
    Jasmine Sum-Yee Yung
    Congping Chen
    Christopher Kai-Shun Leung
    Kai Liu
    Jianan Y. Qu
    Light: Science & Applications, 9
  • [36] Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo
    Qin, Zhongya
    He, Sicong
    Yang, Chao
    Yung, Jasmine Sum-Yee
    Chen, Congping
    Leung, Christopher Kai-Shun
    Liu, Kai
    Qu, Jianan Y.
    LIGHT-SCIENCE & APPLICATIONS, 2020, 9 (01)
  • [37] Femtosecond pulse recoding and regeneration by a two-photon gated periodic diffractive optics
    Nishioka, H
    Tomita, H
    Ueda, K
    ULTRAFAST PHENOMENA XIV, 2005, 79 : 777 - 779
  • [38] Introduction to two-photon microscopy
    Diaspro, A
    MICROSCOPY RESEARCH AND TECHNIQUE, 1999, 47 (03) : 163 - 164
  • [39] Two-photon excitation microscopy
    Diaspro, A
    Chirico, G
    ADVANCES IN IMAGING AND ELECTRON PHYSICS, VOL 126, 2003, 126 : 195 - 286
  • [40] Fundamental of two-photon microscopy
    Nakamura, O
    MICROSCOPY RESEARCH AND TECHNIQUE, 1999, 47 (03) : 165 - 171