Ex vivo phenotyping and potency monitoring of CD19 CAR T cells with a combined flow cytometry and impedance-based real time cell analysis workflow

被引:0
|
作者
Zhao, Li [1 ]
Lei, Ming [2 ]
Ye, Peifang [2 ]
Lu, Yan [2 ]
Ji, Xiaoping [2 ]
Yan, Yu [2 ]
Jachimowicz, Lauren [1 ]
Guenther, Garret [1 ]
Li, Nancy [1 ]
机构
[1] Agilent Technol, San Diego, CA USA
[2] Agilent Technol, Hangzhou, Peoples R China
关键词
D O I
10.1158/1538-7445.AM2020-2145
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
2145
引用
收藏
页数:2
相关论文
共 44 条
  • [11] SINGLE CELL MULTIOMIC ANALYSIS OF CLONAL AND TRANSCRIPTIONAL PROGRAMS OF CD19 CAR T CELLS IN THE IMMUNOTHERAPY RESPONSE
    Murillo, Mercedes Guerrero
    Rill-Hinarejos, Aina
    Trincado, Juan L.
    Bataller, Alex
    Ortiz-Maldonado, Valentin
    Benitez-Ribas, Daniel
    Espanol, Marta
    Gonzalez, Europa Azucena
    Martinez-Cibrian, Nuria
    Marchese, Domenica
    Martin-Martin, Lourdes
    Garcia-Sancho, Alejandro Martin
    Heyn, Holger
    Juan, Manel
    Urbano-Ispizua, Alvaro
    Delgado, Julio
    Orfao, Alberto
    Mereu, Elisabetta
    Bueno, Clara
    Menendez, Pablo
    EXPERIMENTAL HEMATOLOGY, 2024, 137
  • [12] Single cell multiomic analysis of clonal and transcriptional programs of CD19 CAR T cells in the immunotherapy response
    Guerrero-Murillo, M.
    Rill-Hinarejos, A.
    Trincado, J. L.
    Bataller, A.
    Mereu, E.
    Bueno, C.
    Menendez, P.
    KLINISCHE PADIATRIE, 2023, 235 (03): : 195 - 195
  • [13] SINGLE CELL MULTIOMIC ANALYSIS OF CLONAL AND TRANSCRIPTIONAL PROGRAMS OF CD19 CAR T CELLS IN THE IMMUNOTHERAPY RESPONSE
    Guerrero Murillo, Mercedes
    Rill-Hinarejos, Aina
    Trincado, Juan L.
    Bataller, Alex
    Ortiz-Maldonado, Valentin
    Benitez-Ribas, Daniel
    Espanol, Marta
    Azucena Gonzalez, Europa
    Martinez-Cibrian, Nuria
    Marchese, Domenica
    Martin-Martin, Loudres
    Garcia-Sancho, Alejandro Martin
    Heyn, Holger
    Juan, Manel
    Urbano-Ispizua, Alvaro
    Delgado, Julio
    Orfao, Alberto
    Mereu, Elisabetta
    Bueno, Clara
    Menendez, Pablo
    EXPERIMENTAL HEMATOLOGY, 2024, 137
  • [14] Early Real Time Quantification of CD19. CAR T Cells By Flow Cytometry As a Predictive Biomarker for Response in Patients with R/R LBCL
    Blumenberg, Viktoria
    Busch, Galina
    Baumann, Stephan
    Jitschin, Regina
    Winkelmann, Michael
    Hellwig, Konstantin
    Rejeski, Kai
    Froelich, Lisa
    Tast, Benjamin
    Schmidt, Christian
    Hildebrandt, Friederike
    Dekorsy, Franziska
    Schmidkonz, Christian
    Baeuerle, Tobias
    Kunz, Wolfgang G.
    Mueller, Fabian
    Mougiakakos, Dimitrios
    von Bergwelt, Michael
    Buecklein, Veit
    Mackensen, Andreas
    Voelkl, Simon
    Subklewe, Marion
    BLOOD, 2022, 140 : 7522 - 7523
  • [15] Repeat Infusions of CD19 CAR-T Cells: Factors Associated with Response, CAR-T Cell In Vivo Expansion, and Progression-Free Survival
    Gauthier, Jordan
    Bezerra, Evandro Dantas
    Hirayama, Alexandre V.
    Fender, Barbara S.
    Vakil, Aesha
    Steinmetz, Rachel N.
    Chapuis, Aude G.
    Till, Brian G.
    Kiem, Hans-Peter
    Shadman, Mazyar
    Cassaday, Ryan D.
    Riddell, Stanley R.
    Maloney, David G.
    Turtle, Cameron J.
    BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2020, 26 (03) : S267 - S268
  • [16] Preclinical evaluation of cyclophosphamide and fl udarabine combined with CD19 CAR-T in the treatment of B-cell hematologic malignancies in vivo
    Xia, Zhigang
    Tian, Mengyao
    Cheng, Yucai
    Yi, Wenfang
    Du, Zefan
    Li, Tianwen
    Wen, Yuchen
    Li, Lindi
    Liu, Yong
    Chen, Chun
    ONCOLOGY RESEARCH, 2024, 32 (06) : 1109 - 1118
  • [17] CAR T Cell Relapse CD19 Negative ALL Cells Express Integrins and Integrin Blockade Can Prolong Survival In Vivo
    Kim, Hye Na
    Lee, Solah
    Fousek, Kristen
    Li, Shuangyue
    Kim, Anne
    Watanabe, Junji
    Bonig, Halvard
    Ahmed, Nabil
    Abdel-Azim, Hisham
    Heisterkamp, Nora
    Kim, Yong-Mi
    BLOOD, 2017, 130
  • [18] Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real- Time Cell Analysis
    Wong, Jo-Ern
    Zainal, Nurhafiza
    AbuBakar, Sazaly
    Tan, Kim-Kee
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2023, (195):
  • [19] Factors Associated with Response, CAR-T Cell In Vivo Expansion, and Progression-Free Survival after Repeat Infusions of CD19 CAR-T Cells
    Bezerra, Evandro D.
    Gauthier, Jordan
    Hirayama, Alexandre V.
    Pender, Barbara S.
    Hawkins, Reed M.
    Vakil, Aesha
    Steinmetz, Rachel N.
    Chapuis, Aude G.
    Till, Brian G.
    Kiem, Hans-Peter
    Shadman, Mazyar
    Cassaday, Ryan D.
    Riddell, Stanley R.
    Maloney, David G.
    Turtle, Cameron J.
    BLOOD, 2019, 134
  • [20] Impedance-based drug-resistance characterization of colon cancer cells through real-time cell culture monitoring
    Susana, Fuentes-Velez
    Sharmila, Fagoonee
    Alessandro, Sanginario
    Valentina, Gallo
    Chiara, Riganti
    Marco, Pizzi
    Fiorella, Altruda
    Danilo, Demarchi
    TALANTA, 2021, 222