cDNA cloning of ovine pulmonary SP-A, SP-B, and SP-C: isolation of two different sequences for SP-B

被引:6
|
作者
Pietschmann, SM [1 ]
Pison, U [1 ]
机构
[1] Humboldt Univ, Fac Med, Dept Anesthesiol & Intens Care Med, Klinikum Virchow,Klin Anaesthesiol & Operat Inten, D-13353 Berlin, Germany
关键词
pulmonary surfactant; surfactant apoproteins; deletion; ovine protein sequences; messenger ribonucleic acid; complementary deoxyribonucleic acid;
D O I
10.1152/ajplung.2000.278.4.L765
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Pulmonary surfactant promotes alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. The three surfactant proteins SP-A, SP-B, and SP-C contribute to dynamic surface properties involved during respiration. We have cloned and sequenced the complete cDNAs for ovine SP-A and SP-C and two distinct forms of ovine SP-B cDNAs. The nucleotide sequence of ovine SP-A cDNA consists of 1,901 bp and encodes a protein of 248 amino acids. Ovine SP-C cDNA contains 809 bp, predicting a protein of 190 amino acids. Ovine SP-B is encoded by two mRNA species, which differ by a 69-bp in-frame deletion in the region coding for the active airway protein. The larger SP-B cDNA comprises 1,660 bp, encoding a putative protein of 374 amino acids. With the sequences reported, a more complete analysis of surfactant regulation and the determination of their physiological function in vivo will be enabled.
引用
收藏
页码:L765 / L778
页数:14
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