Capillary monoliths for the analysis of nucleic acids by high-performance liquid chromatography-electrospray ionization mass spectrometry

被引：99
|
作者：
Oberacher, H
Huber, CG
机构：
[1] Univ Saarland, D-66041 Saarbrucken, Germany
[2] Univ Innsbruck, Inst Analyt Chem & Radiochem, A-6020 Innsbruck, Austria
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2002年 / 21卷 / 03期
基金：
奥地利科学基金会;
关键词：
nucleic acids; ohgonucleotides; ion-pair reversed-phase liquid chromatography; monolithic capillary columns; electrospray ionization-mass spectrometry (ESI-MS);
D O I：
10.1016/S0165-9936(02)00304-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Monolithic capillary columns prepared by copolymerization of styrene and divinylbenzene inside a 200 mum i.d. fused silica capillary allow the rapid and highly efficient separation of single- and double-stranded DNA by ion-pair reversed-phase high-performance liquid chromatography. Hyphenation to electrospray ionization-mass spectrometry can be readily achieved. (C) 2002 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：166 / 174
页数：9
相关论文
共 50 条
  • [1] Polystyrene/divinylbenzene based monolithic and encapsulated capillary columns for the analysis of nucleic acids by high-performance liquid chromatography-electrospray ionization mass spectrometry
    Huck, CW
    Bakry, R
    Bonn, GK
    [J]. ENGINEERING IN LIFE SCIENCES, 2005, 5 (05): : 431 - 435
  • [2] High-performance liquid chromatography-electrospray ionization mass spectrometry of single- and double-stranded nucleic acids using monolithic capillary columns
    Premstaller, A
    Oberacher, H
    Huber, CG
    [J]. ANALYTICAL CHEMISTRY, 2000, 72 (18) : 4386 - 4393
  • [3] Analysis of Paeoniae Radix by High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
    Xinfeng Zhao
    Yuqing Sun
    [J]. Analytical Sciences, 2003, 19 : 1313 - 1315
  • [4] Analysis of Paeoniae Radix by high-performance liquid chromatography-electrospray ionization-mass spectrometry
    Zhao, XF
    Sun, YQ
    [J]. ANALYTICAL SCIENCES, 2003, 19 (09) : 1313 - 1315
  • [5] Capillary high-performance liquid chromatography-electrospray ionization mass spectrometry using monolithic columns and carbon fiber electrospray ionization emitters
    Ro, KW
    Liu, H
    Busman, M
    Knapp, DR
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2004, 1047 (01) : 49 - 57
  • [6] High-performance liquid chromatography-electrospray ionization mass spectrometry using monolithic capillary columns for proteomic studies
    Premstaller, A
    Oberacher, H
    Walcher, W
    Timperio, AM
    Zolla, L
    Chervet, JP
    Cavusoglu, N
    van Dorsselaer, A
    Huber, CG
    [J]. ANALYTICAL CHEMISTRY, 2001, 73 (11) : 2390 - +
  • [7] Quantification of zolmitriptan in plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry
    Zhang, ZJ
    Xu, FG
    Tian, Y
    Li, W
    Mao, GG
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 813 (1-2): : 227 - 233
  • [8] Sheath liquid effects in capillary high-performance liquid chromatography-electrospray mass spectrometry of oligonucleotides
    Huber, CG
    Krajete, A
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 870 (1-2) : 413 - 424
  • [9] Evaluation of volatile eluents and electrolytes for high-performance liquid chromatography-electrospray ionization mass spectrometry and capillary electrophoresis-electrospray ionization mass spectrometry of proteins - I. Liquid chromatography
    Huber, CG
    Premstaller, A
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 849 (01) : 161 - 173
  • [10] ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY
    Li, Jinhua
    Cai, Zongwei
    Xu, Shoufang
    Liao, Chunyang
    Song, Xingliang
    Chen, Lingxin
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2011, 34 (15) : 1578 - 1593
12345