Taking the ruler to the jungle: single-molecule FRET for understanding biomolecular structure and dynamics in live cells

被引：64

作者：

Sustarsic, Marko ^{[1
]}

Kapanidis, Achillefs N. ^{[1
]}

机构：

[1] Univ Oxford, Dept Phys, Clarendon Lab, Biol Phys Res Grp, Oxford OX1 3PU, England

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2015年 / 34卷

基金：

欧洲研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

RESONANCE ENERGY-TRANSFER; GREEN FLUORESCENT PROTEIN; DIELS-ALDER REACTIONS; LIVING CELLS; IN-VIVO; ELECTROPORATED MOLECULES; LABELED PROTEINS; PROBES; SPECTROSCOPY; AUTOFLUORESCENCE;

D O I：

10.1016/j.sbi.2015.07.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule Forster resonance energy transfer (smFRET) serves as a molecular ruler that is ideally posed to study static and dynamic heterogeneity in living cells. Observing smFRET in cells requires appropriately integrated labeling, internalization and imaging strategies, and significant progress has been made towards that goal. Pioneering studies have demonstrated am FRET detection in both prokaryotic and eukaryotic systems, using both wide-field and confocal microscopies, and have started to answer exciting biological questions. We anticipate that future technical developments will open the door to smFRET for the study of structure, conformational changes and kinetics of biomolecules in living cells.

引用

页码：52 / 59

页数：8

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