Preconcentration and Determination of Cabergoline Using the Green Practical Solvent Bar Liquid Phase Microextraction Technique in Biological Fluids

Background: Cabergoline, used for treating hyperprolactinemia, is hard to detect and determine in biological samples due to its dosage form. Materials and Methods: The trend hollow fiber mediating technique was used for preconcentration and extraction of cabergoline using the solvent bar and HPLC-UV. The parameter affecting the efficiency of this technique was optimized. The pH gradient was adjusted in accordance with the pKa of cabergoline. Other factors such as extraction time, stirring rate, the organic solvent, and salt addition were optimized based on the highest area obtained from HPLC-UV. Results and Discussion: Cabergoline was determined with a good correlation coefficient (r(2) = 0.989), within the linearity range. 0.02ng mL-1 was the limit of detection for cabergoline and the Intra- and inter-day relative standard deviations were no more than 3.2% and 4.6%, respectively. This protocol offered a simple, sensitive, cost effective, and green method with great extraction efficiency for the detection and determination of cabergoline. Conclusion: Eventually, the presented method was proved applicable for human and urine samples.