Immobilization of Fibrinogen Antibody on Self-Assembled Gold Monolayers for Immunosensor Applications

被引：5
|
作者：
Cho, Hongsik [1 ,2 ,3 ]
Zook, Justin [4 ]
Banner, Todd
Park, Sang-Hyug [5 ]
Min, Byoung-Hyun [6 ,7 ,8 ]
Hasty, Karen A. [1 ,2 ,3 ]
Pinkhassik, Eugene
Lindner, Erno [4 ]
机构：
[1] Univ Tennessee, Hlth Sci Ctr, Dept Orthpaed Surg & Biomed Engn, Memphis, TN 38104 USA
[2] Campbell Clinn, Dept Orthopaed Surg, Memphis, TN 38104 USA
[3] Vet Affairs Med Ctr, Memphis, TN 38104 USA
[4] Univ Memphis, Dept Biomed Engn, Memphis, TN 38152 USA
[5] Jungwon Univ, Dept Biomed Engn, Geosan 367700, South Korea
[6] Ajou Univ, Sch Med, Dept Orthopead Surg, Suwon 443749, South Korea
[7] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea
[8] Ajou Univ, Med Ctr, Cell Therapy Ctr, Suwon 443749, South Korea
来源：
TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2014年 / 11卷 / 01期
基金：
美国国家科学基金会;
关键词：
self-assembled monolayers (SAMs); anti-fibrinogen; immunosensors; enzyme-linked immuno sorbent assay (ELISA); ellipsometry; atomic force microscopy (AFM); gold monolayer; antibody immobilization; BIOSENSING APPLICATIONS; PLASMA-FIBRINOGEN; SURFACE; NANOTECHNOLOGY; PROTEINS;
D O I：
10.1007/s13770-013-1119-4
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Self-assembled gold monolayers offer several advantages for the realization of novel modified electrodes for biosensor applications. This is due to their ability to decrease non-specific adsorption and provide for covalent attachment of biomolecules. Surfaces for these applications require the precise control of ligand density, the ability to immobilize ligands, and in situ-modulation of ligand activity. In this study, we focused our studies on the immobilization of antibody on a gold monolayer surface. We self-assembled thioctic acid onto the gold surface as an anchor point for the immobilization of anti-fibrinogen onto the surface. The modifications to the gold surface were characterized by ELISA, ellipsometry, and AFM.
引用
收藏
页码：10 / 15
页数：6
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