A MEMS based amperometric immunosensor with self-assembled monolayers immobilization technique

被引：0
|
作者：
Xu, Yuanyuan [1 ]
Bian, Chao [1 ]
Chen, Shaofeng [1 ]
Xia, Shanhong [1 ]
机构：
[1] Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China
来源：
RARE METAL MATERIALS AND ENGINEERING | 2006年 / 35卷
关键词：
amperometric immunosensor; microelectromechanical systems; self-assembled monolayers; gold nanoparticles; protein G; human immunoglobulin;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Based on MEMS technology, immunosensor with an "Au, Pt, Pt" three-microelectrode system enclosed in a SU-8 micro pool was fabricated. Employing SAMs technique, the Au electrode was modified by cysteamine (Cys) to assemble gold nanoparticles (nanogold) layer, subsequently, a layer of protein G (PG) was immobilized on nanogold layer to further capture antibody orientedly. Compared with the immunosensors using bulky gold electrode and direct PG binding to electrode immobilization technique for antibody, it has attractive advantages, such as miniaturization, good compatibility, broad linear range for human immunoglobulin (HIgG) and easy to be designed into array.
引用
收藏
页码：344 / 345
页数：2
相关论文
共 50 条
  • [1] A MEMS Based Amperometric Immunosensor with Self-assembled Monolayers Immobilization Technique
    Yuanyuan Xu Chao Bian Shaofeng Chen Shanhong Xia (State Key Laboratory of Transducer Technology
    [J]. 稀有金属材料与工程, 2006, (S3) : 344 - 345
  • [2] Immobilization of fibrinogen antibody on self-assembled gold monolayers for immunosensor applications
    Hongsik Cho
    Justin Zook
    Todd Banner
    Sang-Hyug Park
    Byoung-Hyun Min
    Karen A. Hasty
    Eugene Pinkhassik
    Erno Lindner
    [J]. Tissue Engineering and Regenerative Medicine, 2014, 11 : 10 - 15
  • [3] Immobilization of Fibrinogen Antibody on Self-Assembled Gold Monolayers for Immunosensor Applications
    Cho, Hongsik
    Zook, Justin
    Banner, Todd
    Park, Sang-Hyug
    Min, Byoung-Hyun
    Hasty, Karen A.
    Pinkhassik, Eugene
    Lindner, Erno
    [J]. TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2014, 11 (01) : 10 - 15
  • [4] A MEMS based amperometric detector for E-Coli bacteria using self-assembled monolayers
    Gau, JJ
    Lan, EH
    Dunn, B
    Ho, CM
    Woo, JCS
    [J]. BIOSENSORS & BIOELECTRONICS, 2001, 16 (9-12): : 745 - 755
  • [5] A microelectronic technology based amperometric immunosensor for α-fetoprotein using mixed self-assembled monolayers and gold nanoparticles
    Xu, YY
    Chao, BA
    Chen, SF
    Xia, SH
    [J]. ANALYTICA CHIMICA ACTA, 2006, 561 (1-2) : 48 - 54
  • [6] Immobilization of proteins on self-assembled monolayers
    Wadu-Mesthrige, K
    Amro, NA
    Liu, GY
    [J]. SCANNING, 2000, 22 (06) : 380 - 388
  • [7] Self-assembled monolayers with latent aldehydes for protein immobilization
    Hahn, Christoph D.
    Leitner, Christa
    Weinbrenner, Theo
    Schlapak, Robert
    Tinazli, Ali
    Tampe, Robert
    Lackner, Bernd
    Steindl, Christian
    Hinterdorfer, Peter
    Gruber, Hermann J.
    Hoelzl, Martin
    [J]. BIOCONJUGATE CHEMISTRY, 2007, 18 (01) : 247 - 253
  • [8] An Electrochemical Immunosensor for Detection of Staphylococcus aureus Bacteria Based on Immobilization of Antibodies on Self-Assembled Monolayers-Functionalized Gold Electrode
    Braiek, Mohamed
    Rokbani, Karima Bekir
    Chrouda, Amani
    Mrabet, Bechir
    Bakhrouf, Amina
    Maaref, Abderrazak
    Jaffrezic-Renault, Nicole
    [J]. BIOSENSORS-BASEL, 2012, 2 (04): : 417 - 426
  • [9] Sulfur based self-assembled monolayers (SAM's) on piezoelectric crystals for immunosensor development
    Vaughan, RD
    O'Sullivan, CK
    Guilbault, GG
    [J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1999, 364 (1-2): : 54 - 57
  • [10] Sulfur based self-assembled monolayers (SAM’s) on piezoelectric crystals for immunosensor development
    R. D. Vaughan
    C. K. O’Sullivan
    G. G. Guilbault
    [J]. Fresenius' Journal of Analytical Chemistry, 1999, 364 : 54 - 57
12345