Demonstration of mRNA for five species of cytochrome P450 in human bone marrow, bone marrow-derived macrophages and human haemopoietic cell lines

被引：27
|
作者：
Hodges, VM [1 ]
Molloy, GY [1 ]
Wickramasinghe, SN [1 ]
机构：
[1] Imperial Coll, Sch Med, Dept Haematol, London W2 1PG, England
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 2000年 / 108卷 / 01期
关键词：
cytochrome P450s; macrophages; bone marrow; haemopoietic cell lines; mRNA expression;
D O I：
10.1046/j.1365-2141.2000.01816.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The expression of mRNA for five cytochrome P450s (CYP1A1, 2A6/7, 2D6, 2E1 and 3A4) was studied in human bone marrow, bone-marrow-derived macrophages and blood monocyte-derived macrophages. Reverse transcriptase polymerase chain reaction (RT-PCR) detected expression of all Eve CYPs in each of these cell populations, All five CYPs were also expressed in the haemopoietic cell lines HL-60 and HEL and in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines. The data suggest that bone marrow macrophages and probably other bone marrow cell types are capable of metabolizing xenobiotics. This metabolic potential may play a role in the bone marrow damage induced by some drugs and chemicals.
引用
收藏
页码：151 / 156
页数：6
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