Demonstration of mRNA for five species of cytochrome P450 in human bone marrow, bone marrow-derived macrophages and human haemopoietic cell lines

被引：27
|
作者：
Hodges, VM [1 ]
Molloy, GY [1 ]
Wickramasinghe, SN [1 ]
机构：
[1] Imperial Coll, Sch Med, Dept Haematol, London W2 1PG, England
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 2000年 / 108卷 / 01期
关键词：
cytochrome P450s; macrophages; bone marrow; haemopoietic cell lines; mRNA expression;
D O I：
10.1046/j.1365-2141.2000.01816.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The expression of mRNA for five cytochrome P450s (CYP1A1, 2A6/7, 2D6, 2E1 and 3A4) was studied in human bone marrow, bone-marrow-derived macrophages and blood monocyte-derived macrophages. Reverse transcriptase polymerase chain reaction (RT-PCR) detected expression of all Eve CYPs in each of these cell populations, All five CYPs were also expressed in the haemopoietic cell lines HL-60 and HEL and in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell lines. The data suggest that bone marrow macrophages and probably other bone marrow cell types are capable of metabolizing xenobiotics. This metabolic potential may play a role in the bone marrow damage induced by some drugs and chemicals.
引用
收藏
页码：151 / 156
页数：6
相关论文
共 50 条
  • [1] Immunophenotyping of human bone marrow-derived macrophages
    Koller, M
    Willheim, M
    Krugluger, W
    Kurz, M
    Hocker, P
    Forster, O
    BoltzNitulescu, G
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1996, 43 (06) : 626 - 632
  • [2] Human bone marrow niche chemoprotection mediated by cytochrome p450 enzymes
    Alonso, Salvador
    Su, Meng
    Jones, Jace W.
    Ganguly, Sudipto
    Kane, Maureen A.
    Jones, Richard J.
    Ghiaur, Gabriel
    ONCOTARGET, 2015, 6 (17) : 14905 - 14912
  • [3] PREPARATION AND CHARACTERIZATION OF HUMAN-BONE MARROW-DERIVED MACROPHAGES
    HUME, DA
    ALLAN, W
    GOLDER, J
    STEPHENS, RW
    DOE, WF
    WARREN, HS
    JOURNAL OF LEUKOCYTE BIOLOGY, 1985, 38 (04) : 541 - 552
  • [4] Pig Bone Marrow-Derived Macrophages Resemble Human Macrophages in Their Response to Bacterial Lipopolysaccharide
    Kapetanovic, Ronan
    Fairbairn, Lynsey
    Beraldi, Dario
    Sester, David P.
    Archibald, Alan L.
    Tuggle, Christopher K.
    Hume, David A.
    JOURNAL OF IMMUNOLOGY, 2012, 188 (07): : 3382 - 3394
  • [5] Murine bone marrow-derived macrophages constitute feeder cells for human B cell hybridomas
    Hoffmann, P
    JimenezDiaz, M
    Weckesser, J
    Bessler, WG
    JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 196 (01) : 85 - 91
  • [6] A Human Bone Marrow-Derived Stromal Cell Population with Hemogenic Potential
    Mokhtari, Saloomeh
    Colletti, Evan Joseph
    Sanada, Chad
    Lamar, Zanetta S.
    Simmons, Paul J.
    Atala, Anthony
    Krause, Diane S.
    Zanjani, Esmail D.
    Porada, Christopher D.
    Almeida-Porada, Graca
    BLOOD, 2015, 126 (23)
  • [7] Bone marrow-derived cells in mouse and human cornea
    Yamagami, S
    Usui, T
    Amano, S
    Ebihara, N
    CORNEA, 2005, 24 (08) : S71 - S74
  • [8] Human bone marrow-derived mitogenic stimulation selective for bone marrow metastatic tumor cells
    Hahn, T
    Or, R
    Segall, H
    Karov, Y
    EUROPEAN CYTOKINE NETWORK, 1998, 9 (03) : 506 - 506
  • [9] MicroRNA Expression in Bone Marrow-Derived Human MSCs
    Bellayr, Ian H.
    Catalano, Jennifer G.
    Puri, Raj K.
    MOLECULAR THERAPY, 2016, 24 : S96 - S96
  • [10] Expression of cytochrome P450 and glutathione S-transferase in human bone marrow mesenchymal stem cells
    Esmaeli, Shahnaz
    Allameh, Abdolamir
    Aleagha, Mohammad Sajad Emami
    Kazemnejad, Somaieh
    Soleimani, Masoud
    IRANIAN JOURNAL OF BIOTECHNOLOGY, 2012, 10 (04) : 270 - 274
12345