Cytotoxic Effect of Microbial Biosurfactants Against Human Embryonic Kidney Cancerous Cell: HEK-293 and Their Possible Role in Apoptosis

被引：5
|
作者：
Pradhan, Arun Kumar [1 ,2 ]
Pradhan, Nilotpala [1 ,2 ]
Mohapatra, Purusottam [4 ]
Kundu, Chanakya Nath [4 ]
Panda, Prasanna Kumar [2 ]
Mishra, Barada Kanta [3 ]
机构：
[1] CSIR Inst Minerals & Mat Technol, Acad Sci & Innovat Res, Bhubaneswar 751013, Orissa, India
[2] CSIR Inst Minerals & Mat Technol, Bioresources Engn Dept, Bhubaneswar 751013, Orissa, India
[3] CSIR Inst Minerals & Mat Technol, Bhubaneswar 751013, Orissa, India
[4] KIIT Univ, KIIT Sch Biotechnol, Odisha, India
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2014年 / 174卷 / 05期
关键词：
Biosurfactants; Cytotoxic effect; HEK-293; cell; Apoptosis; PURIFICATION;
D O I：
10.1007/s12010-014-1168-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two different microbial biosurfactants S9BS and CHBS were isolated from Lysinibacillus fusiformis S9 and Bacillus tequilensis CH. Cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (HEK-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT) assay and morphological changes were observed under inverted microscope. The biosurfactants exhibited positive cytotoxic effect on HEK-293 cell line. It was found that LC50 of S9BS and CHBS were 75 and 100 mu g ml(-1), respectively. Further cell cycle and apoptosis analysis of biosurfactant-treated HEK-293 cell line were done by FACS. In this study, cytotoxic effect of glycolipid biosurfactant against HEK-293 cell lines is reported for the first time. Mechanism towards increased membrane permeability of biosurfactant-treated cancer cell may be the incorporation of its lipid moiety into the plasma membrane leading to formation of pores and membrane disruption. Hence, these microbial biosurfactants can prove to be significant biomolecule for cancer treatment.
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页码：1850 / 1858
页数：9
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