Placenta and intrauterine infection by Brucella abortus in buffaloes (Bubalus bubalis)

被引:4
|
作者
Sousa, Melina G. S. [1 ]
Salvarani, Felipe M. [1 ]
Bomjardirn, Henrique A. [1 ]
Fonseca, Antonio A., Jr. [2 ]
Preis, Ingred S. [2 ]
Brito, Marilene F. [3 ]
Leite, Romulo C. [1 ]
Barbosa, Jose D. [1 ]
机构
[1] Univ Fed Para UFPA, Fac Med Vet, Inst Med Vet, BR-68741740 Castanhal, PA, Brazil
[2] Lab Nacl Agr Minas Gerais Lanagro MG, BR-33600000 Pedro Leopoldo, MG, Brazil
[3] UFRRJ, Dept Epidemiol & Sande Publ, Inst Vet 4, BR-23890000 Seropedica, RJ, Brazil
来源
PESQUISA VETERINARIA BRASILEIRA | 2015年 / 35卷 / 11期
关键词
Brucellosis; buffaloes; Bubalus bubalis; Brucella abortus; transplacental infection; FLUORESCENCE POLARIZATION ASSAY; WATER-BUFFALO; STRAIN RB51; BACTERIOLOGICAL RESPONSES; BOVINE FETUSES; VACCINE; DIAGNOSIS; CATTLE; PATHOGENESIS; ELISA;
D O I
10.1590/S0100-736X2015001100002
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The objective of this study was to detect Brucella abortus and injuries caused by the bacteria in fetal membranes and fetuses. Twenty buffaloes serologically positive for brucellosis were used and subjected to stamping for collection of material from the closed uterus of several months gestation. Fetal age was determined by ultrasound examination and the size of fetuses was measured at necropsy. The samples were subjected to histopathology and qPCR. From the second month of pregnancy on it was possible to detect the presence of B. bonus DNA in amniotic fluid, allantoic liquid and uterus, and from the fifth month on in placenta, heart, spleen, kidney, lung, intestine, liver and lymph nodes of the fetuses. The main pathological findings were fibrinous suppurative necrotic placentitis, and chronic endometritis.
引用
收藏
页码:882 / 888
页数:7
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