Selective labeling of tag-fused protein by tryptophan-sensitized luminescence of a terbium complex

被引:21
|
作者
Hirayama, Tasuku [1 ]
Taki, Masayasu [1 ,2 ]
Kodan, Atsushi [3 ]
Kato, Hiroaki [4 ]
Yamamoto, Yukio [1 ]
机构
[1] Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Kyoto 6068501, Japan
[2] Kyoto Univ, Grad Sch Global Environm Studies, Sakyo Ku, Kyoto 6068501, Japan
[3] Kyoto Univ, Inst Integrated Cell Mat Sci, Sakyo Ku, Kyoto 6068501, Japan
[4] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Biol Struct, Sakyo Ku, Kyoto 6068501, Japan
关键词
LANTHANIDE-BINDING TAGS; LIVE CELLS; FLUORESCENT-PROBES; HEXAHISTIDINE-TAG; MOLECULES; EMISSION; SYSTEMS; IONS;
D O I
10.1039/b900302a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A Tb3+ complex with two di(2-picolyl)amine (DPA) moieties and an oligo-aspartate peptide containing a tryptophan residue have been designed as a new binding pair for use in protein labeling.
引用
收藏
页码:3196 / 3198
页数:3
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