Physiological and biochemical response of Aedes aegypti tolerance to Bacillus thuringiensis

被引：3

作者：

Wu, Songqing ^{[1
,2
]}

Wu, Wei ^{[1
,2
]}

Zhu, Xiaoli ^{[1
,2
]}

Liu, Zhaoxia ^{[1
,2
]}

Rebeca, Carballar-Lejarazu ^{[3
]}

Fu, Tao ^{[1
,2
]}

Han, Chaoqiang ^{[1
,2
]}

Shao, Ensi ^{[1
,2
]}

Pan, Xiaohong ^{[1
,2
]}

Huang, Zhipeng ^{[1
,2
]}

Xu, Lei ^{[1
,2
]}

Zou, Shuangquan ^{[1
,2
]}

Zhang, Lingling ^{[1
,2
]}

Guan, Xiong ^{[1
,2
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Forestry, Key Lab Biopesticide & Chem Biol, Minist Educ, Fuzhou, Peoples R China

[2] Fujian Agr & Forestry Univ, Fujian Taiwan Joint Ctr Ecol Control Crop Pests, Fuzhou, Peoples R China

[3] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92717 USA

来源：

BIOCONTROL SCIENCE AND TECHNOLOGY | 2016年 / 26卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Bacillus thuringiensis; Aedes aegypti; detoxifying enzymes; mosquito tolerance; GLUTATHIONE S-TRANSFERASES; INSECTICIDE RESISTANCE; FIELD POPULATIONS; MOLECULAR-BASIS; CULEX-QUINQUEFASCIATUS; DETOXIFICATION GENES; AMYLASE INHIBITORS; SUBSP ISRAELENSIS; LARVAL TOLERANCE; ALPHA-AMYLASES;

D O I：

10.1080/09583157.2015.1089216

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus thuringiensis subsp. israelensis (Bti) represents the only eco-friendly bio-degradable insecticide for mosquito-borne disease control. Our research aims to identify if mosquito detoxification enzymes play an important role in Bti tolerance mechanisms in the dengue vector, Aedes aegypti. Several enzymes, such as amylase, cytochromes P450, Na+/K+-ATPase, acetylcholinesterase, protease and glutathione S-transferase (GST) were analysed and level of activity determined in Ae. aegypti larvae after Bti treatment. Bti exposure significantly increased the level of amylase (183.2%) as well as the activity of cytochromes P450 (177.5%), and Na+/K+-ATPase (142.9%). On the other hand, there was a decrease of 8.6% and 11.4% in acetylcholinesterase and GST activity, and no significant effect in the total level of protease activity. We suggest that the variation in amylase, cytochromes P450, Na+/K+-ATPase, acetylcholinesterase, protease and GST activity may be associated with the Bti insecticidal mechanism. This study provides the basis of detoxifying enzymes in Bti tolerance.

引用

页码：227 / 238

页数：12

共 50 条

[41] ROLE OF THE PARASPORAL BODY IN CAUSING TOXICITY OF BACILLUS-THURINGIENSIS TOWARD AEDES-AEGYPTI LARVAE
SAMASANTI, W
PANTUWATANA, S
BHUMIRATANA, A
JOURNAL OF INVERTEBRATE PATHOLOGY, 1982, 39 (01) : 41 - 48
[42] Biosynthesis of silver nanoparticles using Bacillus thuringiensis against dengue vector, Aedes aegypti (Diptera: Culicidae)
A. Najitha Banu
C. Balasubramanian
P. Vinayaga Moorthi
Parasitology Research, 2014, 113 : 311 - 316
[43] THE EFFICACY Bacillus thuringiensis israelensis AND Mesocyclops aspericornis COMBINATION AGAINST LARVAE OF Aedes aegypti IN THE WATER JARS
Yuniarti, R. A.
Damar, T. B.
BULETIN PENELITIAN KESEHATAN, 2008, 36 (01): : 26 - 32
[44] Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae)
Soares-da-Silvaa, Joelma
Soares Pinheiro, Valeria Cristina
Litaiff-Abreu, Eleilza
Polanczyk, Ricardo Antonio
Tadei, Wanderli Pedro
REVISTA BRASILEIRA DE ENTOMOLOGIA, 2015, 59 (01) : 1 - 6
[45] The novel Bacillus thuringiensis HSY204 as a potential bioinsecticide with efficacy against Aedes aegypti larvae
Lin, Qi
Wu, Jiangyu
Feng, Xiao
Yang, Pan
Jaworski, Coline C.
Ahmad, Shakil
Zhang, Wenfei
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2025, 208
[46] Effect of Bacillus thuringiensis ssp. israelensis on the development of Plasmodium gallinaceum in Aedes aegypti (Diptera: Culicidae)
Kala, MK
Gunasekaran, K
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1999, 93 (01): : 89 - 95
[47] TOXICITY OF BACILLUS-THURINGIENSIS VAR ISRAELENSIS CRYSTALS TO AEDES-AEGYPTI LARVAE - CARBONATE REVERSAL
SCHNELL, DJ
NICKERSON, KW
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 45 (05) : 1691 - 1693
[48] Proteomic Response of Aedes aegypti Larvae to Silver/Silver Chloride Nanoparticles Synthesized Using Bacillus thuringiensis subsp. israelensis Metabolites
Chimkhan, Nantipat
Thammasittirong, Sutticha Na-Ranong
Roytrakul, Sittiruk
Krobthong, Sucheewin
Thammasittirong, Anon
INSECTS, 2022, 13 (07)
[49] Biosynthesis of silver nanoparticles using Bacillus thuringiensis against dengue vector, Aedes aegypti (Diptera: Culicidae)
Banu, A. Najitha
Balasubramanian, C.
Moorthi, P. Vinayaga
PARASITOLOGY RESEARCH, 2014, 113 (01) : 311 - 316
[50] Bacillus thuringiensis var. israelensis (Bti) Provides Residual Control of Aedes aegypti in Small Containers
Ritchie, Scott A.
Rapley, Luke P.
Benjamin, Seleena
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2010, 82 (06): : 1053 - 1059

← 1 2 3 4 5 →