Photothermal bleaching in time-lapse photoacoustic microscopy

被引：0
|
作者：
Gao, Liang [1 ]
Wang, Lidai [1 ]
Li, Chiye [1 ]
Garcia-Uribe, Alejandro [1 ]
Wang, Lihong V. [1 ]
机构：
[1] Washington Univ, Dept Biomed Engn, Opt Imaging Lab, St Louis, MO 63130 USA
来源：
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2014 | 2014年 / 8943卷
关键词：
photoacoustic microscopy; photothermal bleaching; FLUORESCENCE MICROSCOPY; GOLD NANOPARTICLES; LIVING CELLS; TOMOGRAPHY; DYNAMICS; SURFACE;
D O I：
10.1117/12.2038564
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We studied the phenomenon of photothermal bleaching - a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time-lapse imaging applications.
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页数：7
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