Genetic transformation of garlic (Allium sativum L.) by particle bombardment

被引：4
|
作者：
Robledo-Paz, A [1 ]
Cabrera-Ponce, JL [1 ]
Villalobos-Arámbula, VM [1 ]
Herrera-Estrella, L [1 ]
Jofre-Garfias, AE [1 ]
机构：
[1] IPN, Ctr Invest & Estudios Avanzados, Dept Ingn Genet Plantas, Irapuato 36500, Gto, Mexico
来源：
HORTSCIENCE | 2004年 / 39卷 / 06期
关键词：
biolistic; transgenic plants; somatic embryogenesis; genetic engineering; tissue culture;
D O I：
10.21273/HORTSCI.39.6.1208
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Microprojectile bombardment was used to introduce DNA into embryogenic callus of garlic (Allium sativum L.) and produce stably transformed garlic plants. Embryogenic calluses, derived from garlic cultivar 'GT96-1', were bombarded with plasmid DNA containing genes coding for hygromycin phosphotransferase and beta-glucuronidase. Putatively transformed calluses were identified in the bombarded tissue after 4 months of selection on 20 mg(.)L(-1) hygromycin B. The transgenic nature of the selected material was demonstrated by GUS histochemical assay and Southern blot hybridization analysis, and twenty transgenic plants were regenerated.
引用
收藏
页码：1208 / 1211
页数：4
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