Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage

被引：4
|
作者：
Liu, Getong [1 ]
Huang, Xin [1 ]
Pu, Qinlin [1 ]
Zhao, Yongyun [1 ]
Du, Feng [1 ]
Dong, Juan [1 ]
Cui, Xin [1 ]
Tang, Zhuo [1 ]
机构：
[1] Univ Chinese Acad Sci, Nat Prod Res Ctr, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 21期
基金：
中国科学院西部之光基金;
关键词：
IN-VITRO SELECTION; METAL-ION; CLEAVING RIBOZYMES; NUX RULE; CATALYSIS; SEQUENCE; RATES; SITE; PHOSPHOROTHIOATE; TRANSITION;
D O I：
10.1039/c7ob00995j
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A new guideline for the construction of hammerhead ribozymes to achieve trans-cleavage of a single-stranded RNA molecule was developed. The sequence rule of the HHRz cleavage site was highly recommended to be "DWH" with an optimal binding arm length of 8-9nt, which diverged from the former rule of "NUX".
引用
收藏
页码：4681 / 4685
页数：5
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