Chemical characterization of milk oligosaccharides of the common brushtail possum (Trichosurus vulpecula)

被引：15
|
作者：
Urashima, Tadasu [1 ]
Fujita, Saori [1 ]
Fukuda, Kenji [1 ]
Nakamura, Tadashi [2 ]
Saito, Tadao [3 ]
Cowan, Phil [4 ]
Messer, Michael [5 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Grad Sch Anim & Food Hyg, Obihiro, Hokkaido 0808555, Japan
[2] Obihiro Univ Agr & Vet Med, Dept Food Sci, Obihiro, Hokkaido 0808555, Japan
[3] Tohoku Univ, Grad Sch Agr, Sendai, Miyagi 9818555, Japan
[4] Landcare Res, Wildlife Ecol & Management, Lincoln 7640, New Zealand
[5] Univ Sydney, Sch Mol Biosci, Sydney, NSW 2006, Australia
来源：
GLYCOCONJUGATE JOURNAL | 2014年 / 31卷 / 05期
关键词：
Common brushtail possum; Milk oligosaccharides; Marsupials; MARSUPIAL-MILK; MACROPUS-EUGENII; TAMMAR WALLABY; LACTATION; COLOSTRUM; PHALANGERIDAE; C-13-NMR;
D O I：
10.1007/s10719-014-9533-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Structural characterizations of marsupial milk oligosaccharides have been performed in only three species: the tammar wallaby, the red kangaroo and the koala. To clarify the homology and heterogeneity of milk oligosaccharides among marsupials, 21 oligosaccharides of the milk carbohydrate fraction of the common brushtail possum were characterized in this study. Neutral and acidic oligosaccharides were separated from the carbohydrate fraction of mid-lactation milk and characterized by H-1-nuclear magnetic resonance spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The structures of the 7 neutral oligosaccharides were Gal(beta 1-3)Gal(beta 1-4)Glc (3'-galactosyllactose), Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc (3", 3'-digalactosyllactose), Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (lacto-N-novopentaose I), Gal(beta 1-3)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (galactosyl lacto-N-novopentaose I), Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-3)Gal(beta 1-4)Glc (galactosyl lacto-N-novopentaose II). The structures of the 14 acidic oligosaccharides detected were Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-4)Glc (sialyl 3'-galactosyllactose), Gal(beta 1-3)(O-3-sulfate)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (lacto-N-novopentaose I sulfate a) Gal(beta 1-3)[Gal(beta 1-4)(O-3-sulfate)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (lacto-N-novopentaose I sulfate b), Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-3)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (sialyl lacto-N-novopentaose a), Gal(beta 1-3)(-3-O-sulfate)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc, Gal(beta 1-3)Gal(beta 1-3)[Gal(beta 1-4)(-3-O-sulfate)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc, Gal(beta 1-3)[Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (sialyl lacto-N-novopentaose b), Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Gal(beta 1-3)(-3-O-sulphate)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc, Gal(beta 1-3)(-3-O-sulphate)Gal(beta 1-3)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc, Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)[Gal(beta 1-4)(-3-O-sulphate)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc and Gal(beta 1-3)Gal(beta 1-3)[Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (galactosyl sialyl lacto-N-novopentaose b). No fucosyl oligosaccharides were detected. Galactosyl lacto-N-novopentaose II, lacto-N-novopentaose I sulfate a, lacto-N-novopentaose I sulfate b and galactosyl sialyl lacto-N-novopentaose b are novel oligosaccharides. The results are compared with those of previous studies on marsupial milk oligosaccharides.
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页码：387 / 399
页数：13
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