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Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells
被引:5
|作者:
Yamamoto, Masahiro
[1
]
Nishiyama, Mitsue
[1
]
Iizuka, Seiichi
[1
]
Suzuki, Shigeaki
[2
]
Suzuki, Norihiro
[2
]
Aiso, Sadakazu
[3
]
Nakahara, Jin
[2
]
机构:
[1] Tsumura & Co, Tsumura Res Labs, 3586 Yoshiwara, Inashiki, Ibaraki 3001192, Japan
[2] Keio Univ, Dept Neurol, Sch Med, Tokyo 1600016, Japan
[3] Keio Univ, Dept Anat, Sch Med, Tokyo 1600016, Japan
关键词:
Enteric glial cells;
Enteric nervous system;
Glial fibrillary acidic protein;
S100B;
Smooth muscle cells;
NERVOUS-SYSTEM DEVELOPMENT;
PIG MYENTERIC PLEXUS;
GASTROINTESTINAL-TRACT;
INCREASED EXPRESSION;
CHANNEL CONTRIBUTES;
RAT-BRAIN;
STEM-CELL;
IN-VIVO;
TRPV1;
ACTIVATION;
D O I:
10.3748/wjg.v22.i44.9752
中图分类号:
R57 [消化系及腹部疾病];
学科分类号:
摘要:
AIM To investigate the possible involvement of transient receptor potential vanilloid 1 (TRPV1) in maturation of enteric glial cells (EGCs). METHODS Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus (MP) as well as cultured MP cells and EGCs using TRPV1 knockout (KO) mice. RESULTS We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type (WT) but not KO mice. Expression of glial fibrillary acidic protein (GFAP) immunoreactive signals was lower at postnatal day (PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100B, another EGC/astrocyte-specific marker. CONCLUSION These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility.
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页码:9752 / 9764
页数:13
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