Genetic inactivation of Ide (Leuciscus idus L.) sperm using UV irradiation

被引：0
|
作者：
Kucharczyk, D [1 ]
机构：
[1] Olsztyn Univ Agr & Technol, Dept Fisheries, PL-10718 Olsztyn Kortowo, Poland
来源：
CYTOBIOS | 1999年 / 99卷 / 392期
关键词：
genome inactivation; haploid; spontaneous gynogenotes; UV irradiation; silver staining; Leuciscus idus;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Ide (Leuciscus idus L.) sperm diluted with 0.85% NaCl in a ratio of 1:9 was genetically inactivated by ultraviolet (UV) irradiation doses ranging from 384 to 3,456 J m(-2). The most effective dose, expressed as a high percentage of hatched haploid embryos, was 1,920 J m(-2). The UV treatment did not kill any spermatozoa, and afterwards the activated spermatozoa showed a high percentage of motility. In the majority of groups treated the spontaneous gynogenotes were recorded and verified using genetic markers.
引用
收藏
页码：149 / 158
页数：10
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