Rapid Quantification of Efavirenz in Human Plasma by Electrospray Ionization Tandem Mass Spectrometry

被引：3
|
作者：
Kailasa, Suresh Kumar [1 ,2 ,3 ]
Wu, Hui-Fen [1 ,2 ,4 ,5 ,6 ]
机构：
[1] Natl Sun Yat Sen Univ, Dept Chem, Kaohsiung 80424, Taiwan
[2] Natl Sun Yat Sen Univ, Ctr Nanosci & Nanotechnol, Kaohsiung 80424, Taiwan
[3] SV Natl Inst Technol, Dept Chem, Surat 395007, India
[4] Natl Sun Yat Sen Univ, Doctoral Degree Program Marine Biotechnol, Kaohsiung 80424, Taiwan
[5] Kaohsiung Med Univ, Coll Pharm, Sch Pharm, Kaohsiung 806, Taiwan
[6] Natl Sun Yat Sen Univ, Inst Med Sci & Technol, Kaohsiung 80424, Taiwan
来源：
JOURNAL OF THE CHINESE CHEMICAL SOCIETY | 2014年 / 61卷 / 04期
关键词：
Efavirenz; Human plasma; ESI-MS; PERFORMANCE LIQUID-CHROMATOGRAPHY; ANTI-HIV DRUGS; ANTIRETROVIRAL THERAPY; AFFINITY PROBES; RITONAVIR; LOPINAVIR; INFECTION; INDINAVIR; MATRIX; AMPRENAVIR;
D O I：
10.1002/jccs.201300359
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report a rapid and sensitive method for detecting efavirenz in human plasma by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Detection of efavirenz in human plasma samples was confirmed by acquiring MS/MS of protonated efavirenz at m/z 316.5 to yield the characteristic fragment ions at m/z 298, 274, 272, 251, 246, 237, 232, 203 and 168, respectively. This approach provided good linearity for the quantification of efavirenz (R-2 = approximate to 0.9989), with good precision (RSD >10%) and limit of detection (0.20 nM). This method can be widely applied for precise quantitative analysis of a variety of drugs in clinical samples.
引用
收藏
页码：437 / 441
页数：5
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