The effects of Y-27632 on pial microvessels during global brain ischemia and reperfusion in rabbits

被引:11
|
作者
Shintani, Noriyuki [1 ]
Ishiyama, Tadahiko [1 ]
Kotoda, Masakazu [2 ]
Asano, Nobumasa [2 ]
Sessler, Daniel I. [3 ]
Matsukawa, Takashi [2 ]
机构
[1] Univ Yamanashi Hosp, Surg Ctr, Chuo Ku, 1110 Shimokato, Yamanashi 4093898, Japan
[2] Univ Yamanashi, Dept Anesthesiol, Fac Med, Chuo Ku, 1110 Shimokato, Yamanashi 4093898, Japan
[3] Cleveland Clin, Inst Anesthesiol, Dept Outcomes Res, Cleveland, OH 44106 USA
来源
BMC ANESTHESIOLOGY | 2017年 / 17卷
关键词
Y-27632; Global brain ischemia-reperfusion; Cranial window; FOCAL CEREBRAL-ISCHEMIA; RHO-KINASE INHIBITOR; BLOOD-FLOW; SEVOFLURANE; PROPOFOL; ACTIVATION; ANESTHESIA;
D O I
10.1186/s12871-017-0331-5
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Background: Global brain ischemia-reperfusion during propofol anesthesia provokes persistent cerebral pial constriction. Constriction is likely mediated by Rho-kinase. Cerebral vasoconstriction possibly exacerbates ischemic brain injury. Because Y-27632 is a potent Rho-kinase inhibitor, it should be necessary to evaluate its effects on cerebral pial vessels during ischemia-reperfusion period. We therefore tested the hypotheses that Y-27632 dilates cerebral pial arterioles after the ischemia-reperfusion injury, and evaluated the time-course of cerebral pial arteriolar status after the ischemia-reperfusion. Methods: Japanese white rabbits were anesthetized with propofol, and a closed cranial window inserted over the left hemisphere. Global brain ischemia was produced by clamping the brachiocephalic, left common carotid, and left subclavian arteries for 15 min. Rabbits were assigned to cranial window perfusion with: (1) artificial cerebrospinal fluid (Control group, n = 7); (2) topical infusion of Y-27632 10(-6) mol . L-1 for 30 min before the initiation of global brain ischemia (Pre group, n = 7); (3) topical infusion of Y-27632 10(-6) mol . L-1 starting 30 min before ischemia and continuing throughout the study period (Continuous group, n = 7); and, (4) topical infusion of Y-27632 10(-6) mol . L-1 starting 10 min after the ischemia and continuing until the end of the study (Post group, n = 7). Cerebral pial arterial and venule diameters were recorded 30 min before ischemia, just before arterial clamping, 10 min after clamping, and 5, 10, 20, 40, 60, 80, 100, and 120 min after unclamping. Results: Mean arterial blood pressure and blood glucose concentration increased significantly after global brain ischemia except in the Continuous group. In the Pre and Continuous groups, topical application of Y-27632 produced dilation of large (mean 18-19%) and small (mean; 25-29%) pial arteries, without apparent effect on venules. Compared with the Control and Pre groups, arterioles were significantly dilated during the reperfusion period in the Continuous and Post groups (mean at 120 min: 5-8% in large arterioles and 11-12% in small arterioles). Conclusions: Y-27632 dilated cerebral pial arterioles during reperfusion. Y-27632 may enhance recovery from ischemia by preventing arteriolar vasoconstriction during reperfusion.
引用
收藏
页数:9
相关论文
共 50 条
  • [21] ROCK inhibitor Y-27632 prevents primary graft non-function caused by warm ischemia/reperfusion in rat liver transplantation
    Mizunuma, K
    Ohdan, H
    Tashiro, H
    Fudaba, Y
    Ito, H
    Asahara, T
    TRANSPLANT INTERNATIONAL, 2002, 15 (12) : 623 - 629
  • [22] Direct effects of Rho-kinase inhibitor on pial microvessels in rabbits
    Kotoda, Masakazu
    Ishiyama, Tadahiko
    Shintani, Noriyuki
    Matsukawa, Takashi
    JOURNAL OF ANESTHESIA, 2015, 29 (02) : 186 - 190
  • [23] Direct effects of Rho-kinase inhibitor on pial microvessels in rabbits
    Masakazu Kotoda
    Tadahiko Ishiyama
    Noriyuki Shintani
    Takashi Matsukawa
    Journal of Anesthesia, 2015, 29 : 186 - 190
  • [24] THE INFLUENCE OF RHO-KINASE INHIBITOR - Y-27632 ON CONTRACTION OF HUMAN MESENTERIC ARTERIES INDUCED BY ANG II AFTER ISCHEMIA AND REPERFUSION
    Szadujkis-Szadurski, R.
    Tafil-Klawe, M.
    Szadujkis-Szadurska, K.
    Szadujkis-Szadurski, L.
    Slupski, M.
    Grzesk, G.
    Matusiak, G.
    BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, 2009, 105 : 44 - 44
  • [25] Effects of melatonin and luzindole during ischemia reperfusion injury in rat pial microcirculation
    Lapi, D.
    Vetri, F.
    Vagnani, S.
    Marchiafava, P. L.
    Colantuoni, A.
    JOURNAL OF VASCULAR RESEARCH, 2006, 43 : 36 - 36
  • [26] Global brain ischemia and reperfusion
    White, BC
    Grossman, LI
    ONeil, BJ
    DeGracia, DJ
    Neumar, RW
    Rafols, JA
    Krause, GS
    ANNALS OF EMERGENCY MEDICINE, 1996, 27 (05) : 588 - 594
  • [27] The effects of topical and intravenous JM-1232(-) on cerebral pial microvessels of rabbits
    Ikemoto, Kodai
    Ishiyama, Tadahiko
    Shintani, Noriyuki
    Asano, Nobumasa
    Sessler, Daniel I.
    Matsukawa, Takashi
    BMC ANESTHESIOLOGY, 2015, 15
  • [28] The effects of topical and intravenous JM-1232(-) on cerebral pial microvessels of rabbits
    Kodai Ikemoto
    Tadahiko Ishiyama
    Noriyuki Shintani
    Nobumasa Asano
    Daniel I Sessler
    Takashi Matsukawa
    BMC Anesthesiology, 15
  • [29] Effects of β1-adrenergic receptor blockade on the cerebral microcirculation in the normal state and during global brain ischemia/reperfusion injury in rabbits
    Asano, Nobumasa
    Hishiyama, Sohei
    Ishiyama, Tadahiko
    Kotoda, Masakazu
    Matsukawa, Takashi
    BMC PHARMACOLOGY & TOXICOLOGY, 2020, 21 (01):
  • [30] Effects of β1-adrenergic receptor blockade on the cerebral microcirculation in the normal state and during global brain ischemia/reperfusion injury in rabbits
    Nobumasa Asano
    Sohei Hishiyama
    Tadahiko Ishiyama
    Masakazu Kotoda
    Takashi Matsukawa
    BMC Pharmacology and Toxicology, 21