Intracytoplasmic injection of human sperm into frozen-thawed hamster oocytes

被引：0
|
作者：
Ahmadi, A [1 ]
Bongso, A [1 ]
Ng, SC [1 ]
机构：
[1] NATL UNIV SINGAPORE, DEPT OBSTET & GYNAECOL, SINGAPORE 119074, SINGAPORE
来源：
MEDICAL SCIENCE RESEARCH | 1996年 / 24卷 / 10期
关键词：
intracytoplasmic sperm injection (ICSI); cryopreservation; oocyte; sperm; hamster-ICSI assay;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Superovulated hamster oocytes were frozen by 2 M DMSO. 89% (499/561) of the oocytes survived freezing and thawing. A hamster-ICSI assay was performed to evaluate the ability of frozen-thawed oocytes to develop male pronuclei. 46% (58/127) of the oocytes had male pronuclear formation following injection of single human sperm into the cytoplasm of frozen-thawed oocytes. There was no significant difference in male pronuclear formation as compared with fresh oocytes. This study shows that hamster oocytes conserved their ability to support human sperm head decondensation and male pronuclear formation following freezing-thawing.
引用
收藏
页码：693 / 694
页数：2
相关论文
共 50 条
  • [21] Pregnancies achieved after frozen-thawed pronuclear oocytes obtained by intracytoplasmic sperm injection with spermatozoa extracted from frozen-thawed testicular tissues from non-obstructive azoospermic men
    Al-Hasani, S
    Demirel, LC
    Schöpper, B
    Bals-Pratsch, M
    Nikolettos, N
    Küpker, W
    Ugur, M
    Sturm, R
    Diedrich, K
    HUMAN REPRODUCTION, 1999, 14 (08) : 2031 - 2035
  • [22] Efficiency of Using Frozen-Thawed Testicular Sperm for Multiple Intracytoplasmic Sperm Injections
    Noritaka Fukunaga
    Kosuke Haigo
    Koichi Kyono
    Yasuhisa Araki
    Journal of Assisted Reproduction and Genetics, 2001, 18 : 634 - 637
  • [23] Pregnancy after intracytoplasmic sperm injection of spermatozoa extracted from frozen-thawed testicular biopsy
    Fischer, R
    Baukloh, V
    Naether, OGJ
    Schulze, W
    Salzbrunn, A
    Benson, DM
    HUMAN REPRODUCTION, 1996, 11 (10) : 2197 - 2199
  • [24] Efficiency of using frozen-thawed testicular sperm for multiple intracytoplasmic sperm injections
    Fukunaga, N
    Haigo, K
    Kyono, K
    Araki, Y
    JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 2001, 18 (12) : 634 - 637
  • [25] The proportion of monopronuclear zygote (1PN) after intracytoplasmic sperm injection (ICSI) is significantly higher in frozen-thawed oocytes than fresh oocytes
    Chuang, M. F.
    Peng, Y. M.
    Lee, M. J.
    Lai, H. H.
    Wang, H. L.
    Hsieh, C. L.
    Huang, Y. C.
    HUMAN REPRODUCTION, 2018, 33 : 204 - 204
  • [26] In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa
    Habermann, H
    Seo, R
    Cieslak, J
    Niederberger, C
    Prins, GS
    Ross, L
    FERTILITY AND STERILITY, 2000, 73 (05) : 955 - 960
  • [27] High pregnancy rate following intracytoplasmic sperm injection using frozen-thawed sperm samples of cancer patients
    Safran, A
    Reubinoff, B
    Porat-Katz, A
    Benshushan, A
    Shayovits, A
    Lewin, A
    HUMAN REPRODUCTION, 1999, 14 : 288 - 288
  • [28] Controversy over the use of fresh versus frozen-thawed sperm for in vitro fertilization-intracytoplasmic sperm injection
    Kim, Edward D.
    FERTILITY AND STERILITY, 2014, 101 (02) : 336 - 336
  • [29] Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal-vesicle stage oocytes
    Verheyen, G
    Nagy, Z
    Joris, H
    DeCroo, I
    Tournaye, H
    VanSteirteghem, A
    FERTILITY AND STERILITY, 1997, 67 (01) : 74 - 80
  • [30] Developmental competence in vivo and in vitro of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection with fresh or frozen-thawed spermatozoa
    Choi, YH
    Love, CC
    Love, LB
    Varner, DD
    Brinsko, S
    Hinrichs, K
    REPRODUCTION, 2002, 123 (03) : 455 - 465
12345