A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7

Investigation on Caulerpa chemnitzia polysaccharides led to the finding of a new polysaccharide (CCP). The basic components of CCP were the total sugar (59.18% +/- 0.57%), the uronic acids (36.75% +/- 0.28%) and the sulfate (42.50% +/- 0.42%), in total content. The physicochemical analysis revealed that CCP was a heteropolysaccharide with a molecular weight of 321.6 KDa, and composed of arabinose, fucose, glucose, mannose, galactose, xylose, fructose, ribose, glucuronic acid and galacturonic acid. The immunomodulatory assay showed that CCP played an important role in activating cell viability, the nitric oxide product and cytokines (IL-6 and TNF-alpha) secretion. Furthermore, the transcript-metabolic analysis displayed a total of 7692 differentially expressed genes (DEGs) and 95 differentially accumulated metabolites (DAMs), and revealed that CCP may play an immunomodulatory effect by activating NF-kappa B signaling pathway and arachidonic acid metabolism pathway. These findings will provide a basic understanding to further investigation of Caulerpa polysaccharides.