Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples

被引：13
|
作者：
Muniesa, Ana [1 ]
Ferreira, Chelo [2 ]
Fuertes, Hector [1 ]
Halaihel, Nabil [1 ]
de Blas, Ignacio [1 ]
机构：
[1] Univ Zaragoza, Fac Vet, Dept Anim Pathol, Zaragoza, Spain
[2] Univ Zaragoza, Dept Appl Math, Fac Vet, Zaragoza, Spain
来源：
PLOS ONE | 2014年 / 9卷 / 04期
关键词：
POLYMERASE-CHAIN-REACTION; PCR; VIRUS;
D O I：
10.1371/journal.pone.0093491
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The high sensitivity of qPCR makes it a desirable diagnostic method in epidemiological surveillance programs. However, due to high costs, the use of pooling has been suggested. In this paper, an algorithm based on the Montecarlo method has been designed and implemented. The algorithm had been tested in many different situations, and finally it was validated with a real dataset. Moreover, based on the results obtained and depending on pooling conditions, a drastic decrease of sensitivity is observed.
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页数：5
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