A new simple method for estimation of allelic frequencies using pooled samples

被引：2

作者：

Saadat, Mostafa ^{[1
]}

Qasemian-Talgard, Akram ^{[1
]}

Darvishi, Fatemeh Zahra ^{[1
]}

Taghipour, Nahid ^{[1
]}

Saadat, Iraj ^{[1
]}

机构：

[1] Shiraz Univ, Dept Biol, Coll Sci, Shiraz 7146713565, Iran

来源：

GENE | 2019年 / 703卷

关键词：

Pooled DNA; Polymorphism; Gel electrophoresis; Estimation; SINGLE-NUCLEOTIDE POLYMORPHISMS; DNA SAMPLES; CANCER; RISK;

D O I：

10.1016/j.gene.2019.04.003

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Today several millions polymorphic sites in human genome are well described. Many investigators are studying the association between these polymorphisms and susceptibility to multifactorial traits. These polymorphisms are also used for studying the population's genetic structures. Here, we introduce a new simple one step method for estimating the allelic frequency of polymorphic sites in pooled samples. The method is based on measurement of the intensity of polymorphic bands on agarose gel electrophoresis. This method is very simple, rapid, inexpensive, and is more sensitive compared to the chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method.

引用

页码：13 / 16

页数：4

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