Screening for topoisomerase I binding compounds by high-performance liquid chromatography-mass spectrometry

被引：13
|
作者：
Zhang, H
Gu, Q
Liang, XL
Pan, YJ [1 ]
机构：
[1] Zhejiang Univ, Dept Chem, Hangzhou, Peoples R China
[2] Hangzhou Univ Commerce, Dept Food Sci, Hangzhou, Peoples R China
[3] Zhejiang Univ, Dept Biol, Hangzhou, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2004年 / 329卷 / 02期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.ab.2004.03.057
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new rapid compound screening approach for topoisomerase I binding activity is presented. DNA topoisomerase I is used as a target protein to capture binding compounds from a mixture of combinatorial compounds by bioaffinity ultrafiltration. Using high-performance liquid chromatography combined with electrospray ionization mass spectrometry, small-molecule active compounds were identified. We also have successfully applied this method to identifying compounds from cells grown in culture. (C) 2004 Elsevier Inc. All rights reserved.
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页码：173 / 179
页数：7
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