Screening for topoisomerase I binding compounds by high-performance liquid chromatography-mass spectrometry

被引：13
|
作者：
Zhang, H
Gu, Q
Liang, XL
Pan, YJ [1 ]
机构：
[1] Zhejiang Univ, Dept Chem, Hangzhou, Peoples R China
[2] Hangzhou Univ Commerce, Dept Food Sci, Hangzhou, Peoples R China
[3] Zhejiang Univ, Dept Biol, Hangzhou, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2004年 / 329卷 / 02期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.ab.2004.03.057
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new rapid compound screening approach for topoisomerase I binding activity is presented. DNA topoisomerase I is used as a target protein to capture binding compounds from a mixture of combinatorial compounds by bioaffinity ultrafiltration. Using high-performance liquid chromatography combined with electrospray ionization mass spectrometry, small-molecule active compounds were identified. We also have successfully applied this method to identifying compounds from cells grown in culture. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码：173 / 179
页数：7
相关论文
共 50 条
  • [1] HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY WITH TRANSPORT INTERFACES
    ALCOCK, NJ
    ECKERS, C
    GAMES, DE
    GAMES, MPL
    LANT, MS
    MCDOWALL, MA
    ROSSITER, M
    SMITH, RW
    WESTWOOD, SA
    WONG, HY
    JOURNAL OF CHROMATOGRAPHY, 1982, 251 (02): : 165 - 174
  • [2] High-performance liquid chromatography-mass spectrometry of an osteocalcin derivative
    Vähätalo, H
    Auriola, S
    Lapinjoki, S
    JOURNAL OF CHROMATOGRAPHY A, 1999, 846 (1-2) : 49 - 57
  • [3] High-performance liquid chromatography-mass spectrometry in the clinical laboratory
    Taylor, PJ
    THERAPEUTIC DRUG MONITORING, 2005, 27 (06) : 689 - 693
  • [4] Detection of Nε-monomethyllysine using high-performance liquid chromatography and high-performance liquid chromatography-mass spectrometry
    Kalász, H
    Szücs, Z
    Tihanyi, M
    Szilágyi, A
    Lengyel, J
    JOURNAL OF CHROMATOGRAPHY A, 2005, 1079 (1-2) : 208 - 212
  • [5] Analysis of sulfonated compounds by ion-exchange high-performance liquid chromatography-mass spectrometry
    Socher, G
    Nussbaum, R
    Rissler, K
    Lankmayr, E
    JOURNAL OF CHROMATOGRAPHY A, 2001, 912 (01) : 53 - 60
  • [6] Analysis of aspartyl peptide degradation products by high-performance liquid chromatography and high-performance liquid chromatography-mass spectrometry
    De Boni, S
    Oberthür, C
    Hamburger, M
    Scriba, GKE
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1022 (1-2) : 95 - 102
  • [7] Determination of LSD in urine with high-performance liquid chromatography-mass spectrometry
    Bodin, K
    Svensson, JO
    THERAPEUTIC DRUG MONITORING, 2001, 23 (04) : 389 - 393
  • [8] ANALYSIS OF SELECTED PESTICIDES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
    VOYKSNER, RD
    BURSEY, JT
    PELLIZZARI, ED
    JOURNAL OF CHROMATOGRAPHY, 1984, 312 (NOV): : 221 - 235
  • [9] Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry
    Perelló, J
    Isern, B
    Muñoz, JA
    Valiente, M
    Grases, F
    CHROMATOGRAPHIA, 2004, 60 (5-6) : 265 - 268
  • [10] Analysis of some folate monoglutamates by high-performance liquid chromatography-mass spectrometry. I
    Stokes, P
    Webb, K
    JOURNAL OF CHROMATOGRAPHY A, 1999, 864 (01) : 59 - 67
12345