A Galactose Oxidase Biosensor Based on Graphene Composite Film for the Determination of Galactose and Dihydroxyacetone

被引：16
|
作者：
Xie, Jia [1 ]
Chen, Chao [1 ]
Zhou, Yuanqing [1 ]
Fei, Junjie [1 ]
Ding, Yonglan [1 ]
Zhao, Jia [2 ]
机构：
[1] Xiangtan Univ, Coll Chem, Key Lab Environm Friendly Chem & Applicat, Minist Educ, Xiangtan 411105, Peoples R China
[2] Hunan Agr Univ, Coll Resource & Environm, Environm Sci & Engn, Changsha 410128, Hunan, Peoples R China
来源：
ELECTROANALYSIS | 2016年 / 28卷 / 01期
基金：
高等学校博士学科点专项科研基金;
关键词：
Galactose oxidase; Graphene; Gold nanoparticles; Direct electron transfer; Biosensor; DIRECT ELECTRON-TRANSFER; ELECTROCHEMICAL-BEHAVIOR; ENZYME ELECTRODE; CARBON; ELECTROCATALYSIS; NANOPARTICLES; MEDIATOR; SENSORS;
D O I：
10.1002/elan.201500486
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel amperometric biosensor was developed by immobilizing galactose oxidase (GAox) on poly (L-lactide) stabilized gold nanoparticles (Au@PLLA) modified graphene nano-composite film (RGO-Au@PLLA/GAox). The direct electron transfer (DET) of GAox was achieved. Cyclic voltammetry of the GAox in the RGO-Au@PLLA film revealed a pair of well-defined and quasireversible peaks at about -0.137V (vs. SCE), which is characterized of the Cu(II)/Cu(I) redox couple for the galactose oxidase. Amperometric response was measured as a function for the determination of galactose (GA) and dihydroxyacetone (DHA). The constructed biosensor also possesses good stability, reproducibility and anti-interference ability.
引用
收藏
页码：183 / 188
页数：6
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