Use of Readycult™-LMX for enumeration of total coliforms and Escherichia coli in milk

被引：1
|
作者：
Beloti, V [1 ]
Barros, MDF [1 ]
Nunes, MP [1 ]
de Santana, EHW [1 ]
Nero, LA [1 ]
de Souza, JA [1 ]
机构：
[1] Univ Estadual Londrina, Ctr Ciencias Agr, Dept Vet Prevent Med, Lab Inspecao Prod Origem Anim, BR-86051970 Londrina, PR, Brazil
来源：
BRAZILIAN JOURNAL OF MICROBIOLOGY | 2002年 / 33卷 / 01期
关键词：
Readycult (TM)-LMX; X-GAL; MUG; milk; Escherichia coli; total coliforms;
D O I：
10.1590/S1517-83822002000100010
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Readycult(TM) - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult(TM) - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult(TM) - LMX. The ReadycuItTM - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for total coliforms (r: 0.8224) and for E. coli (r: 0.8603). The two methods yielded similar results, but Readycult(TM) - LMX was easier to use. In addition, results were available as early as 24h.
引用
收藏
页码：49 / 52
页数：4
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