Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method

被引：10
|
作者：
Morihiro, Kunihiko [1 ,2 ,5 ]
Hasegawa, Osamu [2 ]
Kasahara, Yuuya [1 ,2 ]
Mori, Shohei [2 ]
Kasai, Tatsuro [3 ]
Kuwahara, Masayasu [3 ,4 ]
Obika, Satoshi [1 ,2 ]
机构：
[1] Natl Inst Biomed Innovat Hlth & Nutr NIBIOHN, 7-6-8 Saito Asagi, Ibaraki, Osaka 5670085, Japan
[2] Osaka Univ, Grad Sch Pharmaceut Sci, 1-6 Yamadaoka, Suita, Osaka 5650871, Japan
[3] Gunma Univ, Grad Sch Sci & Technol, 1-5-1 Tenjin Cho, Kiryu, Gunma 3768515, Japan
[4] Nihon Univ, Grad Sch Integrated Basic Sci, Setagaya Ku, 3-25-40 Sakurajosui, Tokyo 1568550, Japan
[5] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2021年 / 31卷
基金：
日本学术振兴会;
关键词：
Azobenzene; CE-SELEX; DNA aptamer; DNA polymerase; 5 '-Triphosphate; EQUILIBRIUM MIXTURES; EVOLUTION; BEARING;
D O I：
10.1016/j.bmcl.2020.127607
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Chemically modified aptamers have recently emerged as important materials for nucleic acid based therapeutics and diagnostic tools. Here, we report in vitro evolution of azobenzene-modified DNA aptamers by capillary electrophoresis (CE)-SELEX method. Azobenzene has been considered to be a fascinating functional group due to its trans-cis photo-isomerization property. We harnessed C5-azobenzene-modified 2'-deoxyuridine (dU(Az)) as a azobenzene-tethered unit and subjected it to CE-SELEX with human thrombin. The obtained dU(Az)-modified aptamer showed strong binding affinity toward human thrombin and could be reversibly photo-isomerized by different wavelengths of light. This work demonstrates that CE-SELEX is a powerful method to obtain chemically modified aptamers and dU(Az) is an excellent photo-responsive nucleoside for nucleic acid photo-switches.
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页数：4
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