Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method

Chemically modified aptamers have recently emerged as important materials for nucleic acid based therapeutics and diagnostic tools. Here, we report in vitro evolution of azobenzene-modified DNA aptamers by capillary electrophoresis (CE)-SELEX method. Azobenzene has been considered to be a fascinating functional group due to its trans-cis photo-isomerization property. We harnessed C5-azobenzene-modified 2'-deoxyuridine (dU(Az)) as a azobenzene-tethered unit and subjected it to CE-SELEX with human thrombin. The obtained dU(Az)-modified aptamer showed strong binding affinity toward human thrombin and could be reversibly photo-isomerized by different wavelengths of light. This work demonstrates that CE-SELEX is a powerful method to obtain chemically modified aptamers and dU(Az) is an excellent photo-responsive nucleoside for nucleic acid photo-switches.