Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing

被引：0
|
作者：
Hernandez, Luis D. [1 ]
Lounsberry, Zachary T. [1 ]
Collins, Paul W. [2 ]
Henry, Robert W. [3 ]
Newsome, Seth D. [4 ]
Sacks, Benjamin N. [1 ,5 ]
机构：
[1] Univ Calif Davis, Vet Genet Lab, Mammalian Ecol & Conservat Unit, Davis, CA 95616 USA
[2] Santa Barbara Museum Nat Hist, Santa Barbara, CA 93105 USA
[3] Univ Calif Santa Cruz, Inst Marine Sci, Ctr Ocean Hlth, Long Marine Lab, Santa Cruz, CA 95060 USA
[4] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA
[5] Univ Calif Davis, Sch Vet Med, Dept Populat Hlth & Reprod, Davis, CA 95616 USA
来源：
CONSERVATION GENETICS RESOURCES | 2014年 / 6卷 / 02期
关键词：
Albatross; Illumina; Microsatellite; PAL FINDER; PCR primers; Phoebastria;
D O I：
10.1007/s12686-013-0138-8
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.
引用
收藏
页码：491 / 493
页数：3
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