Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing

被引：0

作者：

Hernandez, Luis D. ^{[1
]}

Lounsberry, Zachary T. ^{[1
]}

Collins, Paul W. ^{[2
]}

Henry, Robert W. ^{[3
]}

Newsome, Seth D. ^{[4
]}

Sacks, Benjamin N. ^{[1
,5
]}

机构：

[1] Univ Calif Davis, Vet Genet Lab, Mammalian Ecol & Conservat Unit, Davis, CA 95616 USA

[2] Santa Barbara Museum Nat Hist, Santa Barbara, CA 93105 USA

[3] Univ Calif Santa Cruz, Inst Marine Sci, Ctr Ocean Hlth, Long Marine Lab, Santa Cruz, CA 95060 USA

[4] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA

[5] Univ Calif Davis, Sch Vet Med, Dept Populat Hlth & Reprod, Davis, CA 95616 USA

来源：

CONSERVATION GENETICS RESOURCES | 2014年 / 6卷 / 02期

关键词：

Albatross; Illumina; Microsatellite; PAL FINDER; PCR primers; Phoebastria;

D O I：

10.1007/s12686-013-0138-8

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.

引用

页码：491 / 493

页数：3

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