Chemical biology of genomic DNA: minimizing PCR bias

被引：6
|
作者：
McInroy, Gordon R. [1 ]
Raiber, Eun-Ang [1 ]
Balasubramanian, Shankar [1 ]
机构：
[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
来源：
CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 81期
基金：
英国工程与自然科学研究理事会;
关键词：
5-HYDROXYMETHYLCYTOSINE; 5-CARBOXYLCYTOSINE; 5-FORMYLCYTOSINE; DEMETHYLATION; TRANSCRIPTION; GLYCOSYLASE; POLYMERASE; LESION;
D O I：
10.1039/c4cc05107f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
引用
收藏
页码：12047 / 12049
页数：3
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