Desulfurization of light gas oil by a novel recombinant strain from Pseudomonas aeruginosa

被引：22
|
作者：
Watanabe, K [1 ]
Noda, K [1 ]
Ohta, Y [1 ]
Maruhashi, K [1 ]
机构：
[1] Japan Cooperat Ctr, Biorefining Proc Lab, Tech Cooperat Dept, Shimizu, Shizuoka 4240037, Japan
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 11期
关键词：
n-alkane; desulfurization; LGO; Pseudomonas aeruginosa; uptake;
D O I：
10.1023/A:1015549600509
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The dsz desulfurization gene cluster from Rhodococcus erythropolis KA2-5-1 was transferred into the chromosomes of Pseudomonas aeruginosa NCIMB 9571 by using a transposon vector. Resting cells of the recombinant strain, PAR41, desulfurized 63 mg sulfur l(-1) of light gas oil (LGO) containing 360 mg S l(-1). The desulfurization activity for LGO by the resting cells of strain PAR41 grown with n-tetradecane (50% v/v) was much higher (10similar to18-fold) than in glucose-grown cells. P. aeruginosa NCIMB 9571 is able to take up water-insoluble compounds from an oil phase which is enhanced by n-alkane.
引用
收藏
页码：897 / 903
页数：7
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