Detecting protein-protein interactions by Xe-129 NMR

被引：7
|
作者：
Zhao, Zhuangyu [1 ]
Roose, Benjamin W. [1 ]
Zemerov, Serge D. [1 ]
Stringer, Madison A. [1 ]
Dmochowski, Ivan J. [1 ]
机构：
[1] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 75期
关键词：
FRAGMENT COMPLEMENTATION; PICOMOLAR SENSITIVITY; LIVING CELLS; IN-VIVO; C-FOS; REPORTER; XENON; BINDING; CRYPTOPHANE; INHIBITORS;
D O I：
10.1039/d0cc02988b
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Detection of protein-protein interactions (PPIs) is limited by current bioanalytical methods. A protein complementation assay (PCA), split TEM-1 beta-lactamase, interacts with xenon at the interface of the TEM-1 fragments. Reconstitution of TEM-1-promoted here by cFos/cJun leucine zipper interaction-gives rise to sensitive(129)Xe NMR signal in bacterial cells.
引用
收藏
页码：11122 / 11125
页数：4
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