Detecting protein-protein interactions by Xe-129 NMR

被引：7

作者：

Zhao, Zhuangyu ^{[1
]}

Roose, Benjamin W. ^{[1
]}

Zemerov, Serge D. ^{[1
]}

Stringer, Madison A. ^{[1
]}

Dmochowski, Ivan J. ^{[1
]}

机构：

[1] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 75期

关键词：

FRAGMENT COMPLEMENTATION; PICOMOLAR SENSITIVITY; LIVING CELLS; IN-VIVO; C-FOS; REPORTER; XENON; BINDING; CRYPTOPHANE; INHIBITORS;

D O I：

10.1039/d0cc02988b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Detection of protein-protein interactions (PPIs) is limited by current bioanalytical methods. A protein complementation assay (PCA), split TEM-1 beta-lactamase, interacts with xenon at the interface of the TEM-1 fragments. Reconstitution of TEM-1-promoted here by cFos/cJun leucine zipper interaction-gives rise to sensitive(129)Xe NMR signal in bacterial cells.

引用

页码：11122 / 11125

页数：4

共 50 条

[1] XE-129 NMR IN POLYMERS
MILLER, JB
RUBBER CHEMISTRY AND TECHNOLOGY, 1993, 66 (03): : 455 - 461
[2] XE-129 NMR AS A PROBE OF POLYMER BLENDS
WALTON, JH
MILLER, JB
ROLAND, CM
JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 1992, 30 (06) : 527 - 532
[3] APPLICATION OF XE-129 NMR TO POLYMER BLENDS
MANSFELD, M
FLOHR, A
VEEMAN, WS
APPLIED MAGNETIC RESONANCE, 1995, 8 (3-4) : 573 - 586
[4] APPLICATION OF XE-129 NMR TO THE CHARACTERIZATION OF ZEOLITES
JOHNSON, DW
GRIFFITHS, L
ZEOLITES, 1987, 7 (05): : 484 - 487
[5] XE-129 NMR INVESTIGATION OF COAL MICROPORES
TSIAO, C
BOTTO, RE
ENERGY & FUELS, 1991, 5 (01) : 87 - 92
[6] A XE-129 NMR-STUDY OF XE LAYERS ON GRAPHON
NEUE, G
ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE NEUE FOLGE, 1987, 152 : 13 - 22
[7] APPLICATION OF THE XE-129 NMR TECHNIQUE .2. XE-129 NMR-STUDY OF XENON DIFFUSION BETWEEN ZEOLITE CRYSTALLITES
CHEN, QJ
FRAISSARD, J
JOURNAL OF PHYSICAL CHEMISTRY, 1992, 96 (04): : 1814 - 1819
[8] OPTICALLY POLARIZED XE-129 IN NMR-SPECTROSCOPY
PIETRASS, T
GAEDE, HC
ADVANCED MATERIALS, 1995, 7 (10) : 826 - &
[9] MICROPOROSITY IN HETEROPOLYOXOMETALATES - A XE-129 NMR-STUDY
BONARDET, JL
MCGARVEY, GB
MOFFAT, JB
FRAISSARD, J
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1993, 72 : 191 - 195
[10] XE-129 NMR AND THE THERMODYNAMIC PARAMETERS OF XENON HYDRATE
DAVIDSON, DW
HANDA, YP
RIPMEESTER, JA
JOURNAL OF PHYSICAL CHEMISTRY, 1986, 90 (24): : 6549 - 6552

← 1 2 3 4 5 →