Cadmium-affected synthesis of exopolysaccharides by rhizosphere bacteria

被引:19
|
作者
Kowalkowski, T. [1 ,2 ]
Krakowska, A. [1 ,2 ]
Zloch, M. [1 ,2 ]
Hrynkiewicz, K. [3 ]
Buszewski, B. [1 ,2 ]
机构
[1] Nicolaus Copernicus Univ, Fac Chem, Gagarina 7, PL-87100 Torun, Poland
[2] Nicolaus Copernicus Univ, Ctr Modern Interdisciplinary Technol, Torun, Poland
[3] Nicolaus Copernicus Univ, Fac Biol & Environm Protect, Dept Microbiol, Torun, Poland
关键词
biopolymers; bioremediation; exopolysaccharide; rhizosphere; stress response; FIELD-FLOW FRACTIONATION; LACTIC-ACID BACTERIA; LIGHT-SCATTERING; POLYSACCHARIDES; NANOPARTICLES; SEPARATION; STRAINS; STRESS; SIZE;
D O I
10.1111/jam.14354
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aim Study is focused on the influence of cadmium addition to growth media on production yield, their size and molecular mass of exopolysaccharides (EPS) synthesized by three rhizosphere bacteria strains. Inhibition of bacterial growth by increasing concentrations of Cd2+ was also analysed. Methods and Results The highest impact of Cd2+ was noticed on the growth of Arthrobacter sp. and Rhizobium metallidurans. Chryseobacterium sp. and Arthrobacter sp. produced significantly lower when compared to R. metallidurans amounts of EPS under the influence of Cd2+. In all bacterial strains both size and molecular mass decreased after addition of Cd2+ to growth media. It causes a change in EPS conformation to more planar, which minimizes the volume of liquid in the interglobular space next to the bacterial wall. Results confirmed strong effect of Cd2+ on the structure and synthesis of bacterial EPS what can be a key factor in the interactions between rhizosphere bacteria and host plants in heavy metal polluted soils. Conclusion This work proves that due to the presence of cadmium ions, the size and conformation of EPS produced by selected bacterial strains is changed to minimize their impact on cell. We suggest that shifting in EPS conformation from bigger globular particles to the smaller planar ones could be one of the probable mechanisms of Cd resistance in metallotolerant bacteria, and finally explain increased efficiency of heavy metal phytoextraction by EPS-producing plant growth-promoting micro-organisms. Significance and Impact of the Study One of the most promising remediation technique for Cd-contaminated areas is the phytoremediation in which rhizosphere bacteria play an important role by protecting plants' roots from toxic condition thus enhancing efficiency of intake. EPS secretion by bacteria is one of the most common mechanisms to protect the cell from impact of unpleasant environmental conditions, for example, toxicity of heavy metals like Cd.
引用
收藏
页码:713 / 723
页数:11
相关论文
共 50 条
  • [31] COLONIZATION POTENTIAL OF BACTERIA IN THE RHIZOSPHERE
    BENNETT, RA
    LYNCH, JM
    CURRENT MICROBIOLOGY, 1981, 6 (03) : 137 - 138
  • [32] THE BEHAVIOR AND TRACKING OF BACTERIA IN THE RHIZOSPHERE
    KLUEPFEL, DA
    ANNUAL REVIEW OF PHYTOPATHOLOGY, 1993, 31 : 441 - 472
  • [33] STUDY OF POLYSACCHARIDES OF RHIZOSPHERE BACTERIA
    KOFLAKOVA, L
    LASIK, J
    FOLIA MICROBIOLOGICA, 1970, 15 (03) : 215 - +
  • [34] Bacteria associated with the corn rhizosphere
    Thuar, AM
    Olmedo, CA
    Andrés, J
    PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY, 1999, 65 : 87 - 90
  • [35] CHITIN BREAKDOWN OF RHIZOSPHERE BACTERIA
    MIHALY, K
    NATURE, 1960, 188 (4746) : 251 - 251
  • [36] OSMOTOLERANCE OF DIAZOTROPHIC RHIZOSPHERE BACTERIA
    HARTMANN, A
    PRABHU, SR
    GALINSKI, EA
    PLANT AND SOIL, 1991, 137 (01) : 105 - 109
  • [37] Chemiqal Behavior of Cadmium in Wheat Rhizosphere
    CHEN NENG-CHANG and CHEN HUAI-MANInstitute of Soil Science
    Pedosphere, 1992, (04) : 363 - 371
  • [38] Synthesis and application of bacterial exopolysaccharides
    Ruijgrok, Gijs
    Wu, Dung-Yeh
    Overkleeft, Herman S.
    Codee, Jeroen D. C.
    CURRENT OPINION IN CHEMICAL BIOLOGY, 2024, 78
  • [39] Root responses to cadmium in the rhizosphere: a review
    Lux, Alexander
    Martinka, Michal
    Vaculik, Marek
    White, Philip J.
    JOURNAL OF EXPERIMENTAL BOTANY, 2011, 62 (01) : 21 - 37
  • [40] LIPIDS IN SYNTHESIS OF LIPOPOLYSACCHARIDES AND EXOPOLYSACCHARIDES
    SUTHERLAND, IW
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1975, 3 (06) : 840 - 843