Maternal CD8+ T-cell depletion alleviates intrauterine inflammation-induced perinatal brain injury

被引：28

作者：

Lei, Jun ^{[1
]}

Xie, Li ^{[1
]}

Zhao, Hongxi ^{[1
]}

Gard, Candice ^{[1
]}

Clemens, Julia L. ^{[1
]}

McLane, Michael W. ^{[1
]}

Feller, Mia C. ^{[1
]}

Ozen, Maide ^{[2
]}

Novak, Christopher ^{[1
]}

Alshehri, Wael ^{[1
]}

Alhejaily, Nader ^{[1
]}

Shabi, Yahya ^{[1
]}

Rosenzweig, Jason M. ^{[1
]}

Facciabene, Andrea ^{[3
]}

Burd, Irina ^{[1
,4
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Gynecol & Obstet, Integrated Res Ctr Fetal Med, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21205 USA

[3] Univ Penn, Sch Med, Dept Obstet & Gynecol, Philadelphia, PA 19104 USA

[4] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2018年 / 79卷 / 05期

关键词：

lipopolysaccharide; lymphocyte trafficking; neurobehavior; placenta; preterm birth; INDUCED PRETERM BIRTH; TUMOR-INFILTRATING LYMPHOCYTES; MOUSE MODEL; INFECTION; RATIO; CHORIOAMNIONITIS; EXPRESSION; INTERFACE; THERAPY; TISSUE;

D O I：

10.1111/aji.12798

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the mechanisms by which CD8(+) T-cell trafficking in placenta contributes to perinatal brain injury by studying effects of maternal CD8(+) T-cell depletion (DEP) in a mouse model of intrauterine inflammation (IUI). Maternal CD8(+) T cells were depleted with anti-CD8(+) antibodies. IUI was induced with lipopolysaccharide (LPS). DEP was confirmed using flow cytometry. Preterm birth rate was evaluated. Offspring neurologic sequelae were assessed by Nissl staining, immune arrays, confirmatory individual TaqMan (R) gene assays, and neurobehavioral tests. DEP did not significantly prevent LPS-induced preterm birth but improved neurobehavioral performance (P<.001) and increased cortical neuronal density (P<.05) in LPS-exposed pups compared to controls. These changes were associated with decreased CCL3 and CXCL10 and increased CCL5 in DEP LPS-exposed mice. We demonstrate that DEP reduces perinatal brain injury following IUI. This supports a role for maternal CD8(+) T-cell trafficking in placenta in mediating perinatal brain injury separate from preterm birth mechanisms.

引用

页数：12

共 50 条

[21] The antigen-specific memory CD8+ T-cell response induced by BCG in cattle resides in the CD8+γ/δTCR-CD45RO+ T-cell population
Hogg, Alison E.
Worth, Andrew
Beverley, Peter
Howard, Christopher J.
Villarreal-Ramos, Bernardo
VACCINE, 2009, 27 (02) : 270 - 279
[22] How are HBV-specific CD8+ T-cell responses induced?
Isogawa, Masanori
Murata, Yasuhiro
Kawashima, Keigo
Tanaka, Yasuhito
FUTURE VIROLOGY, 2018, 13 (12) : 825 - 827
[23] MAF drives CD8+ T-cell exhaustion
Verdeil, Gregory
ONCOIMMUNOLOGY, 2016, 5 (02):
[24] Shaping and reshaping CD8+ T-cell memory
John T. Harty
Vladimir P. Badovinac
Nature Reviews Immunology, 2008, 8 : 107 - 119
[25] Pathways of memory CD8+ T-cell development
Bannard, Oliver
Kraman, Matthew
Fearon, Douglas
EUROPEAN JOURNAL OF IMMUNOLOGY, 2009, 39 (08) : 2083 - 2087
[26] Modelling pathways of CD8+ T-cell differentiation
Yates, Andrew
EUROPEAN JOURNAL OF IMMUNOLOGY, 2009, 39 (01) : 47 - 49
[27] CD8+ CUTANEOUS T-CELL LYMPHOMA - REPLY
URRUTIA, S
PIRIS, MA
ORRADRE, JL
MARTINEZ, B
CRUZ, MA
GARCIAALMAGRO, D
AMERICAN JOURNAL OF DERMATOPATHOLOGY, 1991, 13 (06) : 628 - 629
[28] Molecular Dissection of CD8+ T-Cell Dysfunction
Wang, Chao
Singer, Meromit
Anderson, Ana C.
TRENDS IN IMMUNOLOGY, 2017, 38 (08) : 567 - 576
[29] Hepatic effector CD8+ T-cell dynamics
Matteo Iannacone
Cellular & Molecular Immunology, 2015, 12 : 269 - 272
[30] Reversing CD8+ T-cell exhaustion with DLI
Martin, Paul J.
BLOOD, 2014, 123 (09) : 1289 - 1290

← 1 2 3 4 5 →