Development of a real-time PCR for tomato yellow leaf curl Sardinia virus

被引：0

作者：

Chouchane, S. Gharsallah

Gorsane, F.

Louzir, H.

Nakhla, M. K.

Marrakchi, M.

Fakhfakh, H. ^{[1
]}

机构：

[1] Fac Sci, Mol Genet Immunol & Biotechnol Lab, Tunis 2092, Tunisia

[2] Fac Sci Bizerte, Bizerte, Tunisia

[3] Inst Pasteur, Immunol Lab, Tunis, Tunisia

[4] Univ Wisconsin, Dept Plant Pathol, Madison, WI USA

来源：

ACTA VIROLOGICA | 2006年 / 50卷 / 02期

关键词：

coat-protein gene; real-time PCR; TaqMan (R) chemistry; tomato yellow leaf curl Sardinia virus; SYBR (R) Green I chemistry;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recently, tomato yellow leaf curl disease has become important for the tomato grown both in greenhouse and field conditions in Tunisia. Here, we describe a rapid, specific, reliable, and sensitive real-time PCR, based on TaqMan (R) chemistry, for Tomato yellow leaf curl Sardinia virus (TYLCSV). This method proved suitable for the detection and quantification of this virus in tomato, pepper and bean plants. It detected the virus even in the samples that were negative by conventional assays.

引用

页码：101 / 105

页数：5

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