Fluorometric assay of DNA by its fluorescent reaction with chloroacetic acid

被引：4
|
作者：
Li, YZ [1 ]
Liu, XJ [1 ]
Liu, HF [1 ]
Liu, HT [1 ]
Ci, YX [1 ]
机构：
[1] BEIJING UNIV,DEPT CHEM,BEIJING 100871,PEOPLES R CHINA
来源：
ANALYTICAL SCIENCES | 1997年 / 13卷 / 02期
关键词：
fluorescent reaction; DNA; cytosine; dCMP; chloroacetic acid; triethylamine;
D O I：
10.2116/analsci.13.251
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new fluorescent reaction of DNA with chloroacetic acid in the presence of triethylamine is proposed, and the reaction conditions have been optimized. Based on this fluorescent reaction, a sensitive fluorometric method is described for the determination of DNA and the selective determination of cytosine and dCMP. The detection limits for DNA, cytosine and dCMP were 6.0, 0.4 and 1.4 ng/ml, respectively.
引用
收藏
页码：251 / 254
页数：4
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