NEW MICROSATELLITE MARKERS FOR TRICYRTIS MACRANTHA (CONVALLARIACEAE) AND CROSS-AMPLIFICATION IN CLOSELY RELATED SPECIES

被引：2
|
作者：
Ohki, Noriko [1 ]
Setoguchi, Hiroaki [1 ]
机构：
[1] Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Kyoto 6068501, Japan
来源：
APPLICATIONS IN PLANT SCIENCES | 2013年 / 1卷 / 05期
关键词：
Convallariaceae; cross-amplifi cation; genetic diversity; microsatellite; population structure; Tricyrtis macrantha;
D O I：
10.3732/apps.1200247
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: Microsatellite markers were characterized in Tricyrtis macrantha (sect. Brachycyrtis, Convallariaceae), a vulnerable species endemic to Japan, to investigate its genetic diversity and population structure. Methods and Results: Eleven microsatellite markers were developed. The number of alleles per locus ranged from one to seven, and genetic diversity per locus ranged from 0.142 to 0.812. Four markers were successfully amplified in other species of sect. Brachycyrtis (T. ishiiana, T. ishiiana var. surugensis, and T. macranthopsis). Conclusions: The microsatellite markers can be used to investigate the genetic diversity and population structure of the vulnerable and endangered species of Tricyrtis sect. Brachycyrtis, to aid in the development of conservation strategies for each species.
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页数：3
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