Molecular RHD screening of RhD negative donors can replace standard serological testing for RhD negative donors

被引：19

作者：

Gowland, Peter ^{[1
]}

Gassner, Christoph ^{[2
]}

Hustinx, Hein ^{[1
]}

Stolz, Martin ^{[1
]}

Gottschalk, Jochen ^{[2
]}

Tissot, Jean-Daniel ^{[3
]}

Thierbach, Jutta ^{[4
]}

Maier, Andreas ^{[5
]}

Sigurdardottir, Sonja ^{[2
]}

Still, Franziska ^{[1
]}

Fontana, Stefano ^{[1
]}

Frey, Beat M. ^{[2
]}

Niederhauser, Christoph ^{[1
]}

机构：

[1] Blood Transfus Serv SRC Berne, Bern, Switzerland

[2] Blood Transfus Serv SRC Zurich, Zurich, Switzerland

[3] Blood Transfus Serv SRC Vaud, Epalinges, Switzerland

[4] Blood Transfus Serv SRC Nordost Schweiz, St Gallen, Switzerland

[5] Blood Transfus Serv SRC Innerschweiz, Luzern, Switzerland

来源：

TRANSFUSION AND APHERESIS SCIENCE | 2014年 / 50卷 / 02期

关键词：

RhD; Molecular RHD screening; IAT; ANTI-D IMMUNIZATION; RED-BLOOD-CELLS; WEAK-D; ALLELES; PHENOTYPE; INDIVIDUALS; GENE;

D O I：

10.1016/j.transci.2014.02.009

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This work aims to assess the value of a generalized molecular RHD screening strategy which could replace routine serological screening of weak D by indirect antiglobulin test. Three independent studies were performed at the two Blood Transfusion Services Berne and Zurich. Donors investigated were 652 RhD negative, but RhC and/or RhE positive, 17,391 mainly Rhccee, and 8200 with normal RhCcEe phenotype distribution. In study I single samples, in studies II and III minipools of 24 and 20 donor samples were tested, respectively. Among 26,243 phenotypically RhD negative blood donors, 65 carriers of RHD alleles were identified. Thirty-one of them were redefined as RhD positive. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：163 / 168

页数：6

共 50 条

[21] Implementation of the RHD fetal protocol in RHD negative gestants
Lepe Balsalobre, E.
Rubio Sanchez, R.
Viloria Penas, M. D. M.
Moro Ortiz, A.
CLINICA CHIMICA ACTA, 2019, 493 : S585 - S586
[22] Differences between RhD-negative Africans and RhD-negative Europeans
Daniels, G
Green, C
Smart, E
LANCET, 1997, 350 (9081): : 862 - 863
[23] RHD gene in blood donors phenotyped as D-negative: Clinical implications
Castilho, L.
Paixao, T.
Barros, C.
Credidio, D. C.
Ribeiro, K. A.
Pellegrino, J., Jr.
TRANSFUSION, 2006, 46 (09) : 140A - 140A
[24] Utility of RHD genotyping in donors with weakly-expressed RhD antigens
Johnson, ST
Bell, JA
Graef, JM
Pirelli, KJ
Bellissimo, DB
TRANSFUSION, 2005, 45 (03) : 126A - 126A
[25] RHD/CE genotyping by polymerase chain reaction using sequence-specific primer in RHD negative Korean donors.
Kim, YT
Kim, SB
Lee, YH
Park, SH
Whang, YS
Seo, DH
Cho, YJ
AMERICAN JOURNAL OF HUMAN GENETICS, 1999, 65 (04) : A178 - A178
[26] Screening for DEL phenotype in RhD negative Indians
Kulkarni, Swati
Parchure, Disha S.
Gopalkrishnan, Vidya
Madkaikar, Manisha
JOURNAL OF CLINICAL LABORATORY ANALYSIS, 2018, 32 (03)
[27] Fetal RHD typing -: Is fetal RHD typing in all RhD negative women cost effective?
Szczepura, Ala
Bonsel, Gouke
Krauth, Christian
Osipenko, Leeza
Haverkamp, Alexander
BRITISH MEDICAL JOURNAL, 2008, 336 (7650): : 906 - 906
[28] Anti-RhD prophylaxis for RhD negative pregnant women
Alfirevic, Zarko
Callaghan, Therese
BMJ-BRITISH MEDICAL JOURNAL, 2014, 349
[29] Study of RHD genes amongst RhD negative patients in Xuzhou
Gao, Y.
Meng, X.
Hu, J.
Bi, X.
Zhang, Y.
Xu, P.
Ygeng
Wu, Z.
Wang, S.
Liang, J.
MINERVA BIOTECNOLOGICA, 2015, 27 (04) : 211 - 213
[30] The presence of RhD gene sequences in RhD negative individuals.
DeMarchi, JM
Merrill, A
Cook, HD
Allitto, BA
AMERICAN JOURNAL OF HUMAN GENETICS, 2000, 67 (04) : 43 - 43

← 1 2 3 4 5 →